, 2011), which may represent additional adaptive traits that promote distribution of the plasmid, or its genes, among nosocomial bacteria. chrA gene homologues from plasmids of Pseudomonas Selleckchem 5-Fluoracil sp. (Tauch
et al., 2003) and Comamonas sp. (Ma et al., 2007), as well as from the chromosomes of Ochrobactrum tritici 5bvl1 (Branco et al., 2008), Bacillus cereus SJ1 (He et al., 2010), and Pseudomonas sp. (Petrova et al., 2011), are also located on putative transposable elements. In conclusion, our results showed that chrA gene homologues are frequently found in plasmids of enterobacterial isolates of nosocomial origin and suggest that CrR genes may be transferred among hospital bacteria owing to their location within genetic mobile elements, probably coselected by antibiotic exposure. The present work was partially supported by grants from Coordinación de Investigación Científica (UMSNH; 2.6 and 2.35) and Consejo Nacional de Ciencia y Tecnología, México (Conacyt no. 79190). GGC-F and YMA-N were recipients of postgraduate and graduate fellowships from Conacyt, respectively. “
“In Streptococcus mutans, ComX, an alternative sigma factor, Selleckchem MLN0128 drives the transcription
of the ‘late-competence genes’ required for genetic transformation. ComX activity is modulated by inputs from two signaling pathways, ComDE and ComRS, that respond to the competence-stimulating peptide (CSP) and the SigX-inducing peptide (XIP), respectively. In particular, the comRS, encoding the ComR regulatory protein and the ComS precursor to XIP, functions as the proximal regulatory system for ComX activation. Here, we investigated the individual and combinatorial effects of CSP and XIP on genetic transformation and cell killing of S. mutans. Our transformation results confirm mafosfamide the recent reports by Mashburn-Warren et al. and Desai et al. that XIP functions optimally in a chemically defined medium, whereas its activity is inhibited
when cells are grown in complex medium. Using tandem mass spectrometry (MS/MS) fragmentation, a drastic reduction in XIP levels in ComX-deficient cultures were observed, suggesting a ComX-mediated positive feedback mechanism for XIP synthesis. Our evaluation of cell viability in the presence of 10 μM XIP resulted in killing nearly 82% of the population. The killing activity was shown to be dependent on the presence of comR/S and comX. These results suggest a novel role for XIP as a compelling effector of cell death. This is the first report that demonstrates a role for XIP in cell killing. The acquisition of novel, heritable DNA by genetically competent bacteria not only propagates antibiotic resistance and virulence determinants, but also shapes bacterial genomes contributing to rapid evolutionary changes (Kroll et al., 1988; Seifert et al., 1988; Feil et al., 1999; Cody et al., 2003; Didelot & Maiden, 2010).
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