chrysogenum NRRL1951). We have reported in a previous work that unprocessed proIAT molecules exert a regulatory role generating slow-processing molecules of IAT, thus decreasing the amount CB-5083 nmr of
the active form and the penicillin biosynthetic activity [26]. Therefore, the lack of IAL processing might be another explanation for its lack of activity in P. chrysogenum. However, when we analysed the sequence of this protein, we found that the G102-C103 processing site of IAT is conserved in the IAL (G105-C106). Self-processing of the IAL was confirmed by MALDI-TOF peptide mass spectrometry after SDS-PAGE analysis of the IAL synthesized in E. coli at 26°C. This indicates that the IAL, like the IAT, belongs to the NTN family of proteins, which are capable of self-activation, as it occurs with other NTN amidohydrolases [23, 37]. Despite the proper processing, in vitro phenylacetyl-CoA: Selleckchem GW 572016 6-APA acyltransferase activity was not detected,
proving that misprocessing is not responsible for the lack of activity. A detailed analysis of the IAL sequence showed that the amino acid equivalent to the S309 in the IAT, which has been reported to be required for enzyme activity [38], is not conserved in the IAL of P. chrysogenum (this amino acid has been replaced by N323). However, in the IAL homologue of A. nidulans the amino acid equivalent to the S309 is conserved, indicating that this might be the main reason for the disparity in enzyme activity between the IALs of these two fungi. The S309 is part of the GXS309XG motif present in the P. chrysogenum and A. nidulans IATs and has been previously proposed to be involved in cleavage of phenylacetyl-CoA and binding of the phenylacetyl moiety to form learn more acyl-enzyme molecules [21, 31]. The formation of phenylacetyl-enzyme and other acyl-enzyme molecules has been confirmed in the IAT by mass spectrometry [39]. Although the A. nidulans IAL does not exactly contain the GXSXG motif, the presence of the Ser272, equivalent Meloxicam to the Ser309, may be sufficient for the activity of this enzyme. The availability of the genome of several ascomycetes has revealed
the presence of ial gene homologues in penicillin and non-penicillin producing fungi, whereas the penDE gene homologues are only found in penicillin-producing fungi, such as A. nidulans and A. oryzae. This might indicate that during evolution, a single ancestral gene was duplicated, giving rise to the penDE (or aatA) gene and its paralogue, the ial gene (initially encoding a NTN amidohydrolase not active in P. chrysogenum and with low activity in A. nidulans). The P. chrysogenum IAL and related proteins in other fungi form a separate evolutive clade from IATs (Fig. 7), indicating that they evolved separately. This hypothesis is supported by the presence of duplicated genes encoding putatives IAT and IAL homologues in A. oryzae, which also contains the penicillin gene cluster. From those ascomycetes containing this cluster, only A.
Related posts:
- As described for other fungi [29, 30]
deletion of the P - Improved sensitivity to ionizing radiation was not observed durin
- Similarly, Hales et al (1998) reported the existence of two diff
- The Spanking New DPP-4 research Solution Will Work While You Sleep!
- , 2006) database (Figs 7 and S2) and from previous analyses (Iva