In our experiment, we used a 408-nm excitation wavelength laser

In our experiment, we used a 408-nm excitation wavelength laser. Optical sections were averaged three times to reduce noise. RNase [email protected] for in

vivo fluorescence imaging Male 4-week-old athymic nude mice were purchased SB-715992 chemical structure from Shanghai Slac Laboratory Animal Co. Ltd (Shanghai, China). All experiments that involve animal use were performed in compliance with the relevant laws and institutional guidelines. All animal experiments were approved by the Institutional Animal Care and Use Committee of Shanghai Jiao Tong University (No. SYXK2007-0025). For the establishment of the tumor model, MGC-803 cells were resuspended in PBS, and 2 × 106 cells per site were subcutaneously injected. The tumor nodules had reached a volume of 0.1 to 0.3 cm3 approximately 3 weeks post-injection. For in vivo fluorescence tumor imaging experiments, 100 μl (5 mg/ml) RNase [email protected] aqueous solution was intratumorally injected into the MGC-803 tumor-bearing mice. Time-course fluorescent images (excitation, 500/20 nm; emission, 600/30 nm; integration time, 5 s) were acquired on a Bruker In-Vivo F PRO imaging system (Bruker, Billerica, MA, USA). Results and

discussion Characterization and properties of RNase [email protected] TEM images of the as-prepared RNase [email protected] that were trapped in the dialysis membrane (MW cutoff 1,000) are shown in Figure 1a; the size of the RNase [email protected] varies mainly within 25 to 45 nm with relatively irregular

morphologies. High-resolution TEM image (Figure 1b, the zoomed-in Entinostat image of the area within the circle in Figure 1a) selleck products clearly shows that the particles are actually formed by encapsulating several C-dots within the RNase A film, so we can call them clusters. The clusters can also extremely easily disperse in pure water. In Figure 1c, the average size of C-dot that dispersed out of the dialysis membrane is about 4 nm (Figure 1f) in diameter with nice spherical morphologies (Figure 1d), and the dispersions are also excellent. Lattice spacing of approximately Carbohydrate 0.23 nm clearly displayed in the high-resolution TEM image (Figure 1d) indicates the (100) facet of graphite [30]. Figure 1 TEM and HR-TEM images, XRD pattern, and size distribution of RNase [email protected] (a) TEM image of the as-prepared RNase [email protected] inside the dialysis membrane after dialyzing against pure water. One typical RNase [email protected] cluster is labeled with a black circle. (b) High-resolution TEM (HR-TEM) image of one focused area within the black circle. (c) TEM image of the C-dots outside the dialysis membrane. (d) HR-TEM image of one single C-dot. (e) XRD pattern of RNase [email protected] (f) Size distribution of C-dots. We can reasonably conclude that during the reaction process accelerated by microwave heating, RNase A capped the different numbers of C-dots that cause the different sizes of particles.

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