NCT00244751). Patients were stratified into Ishak stages F2 (n=78), F3 (n=88), or F4 (n=28). Paired liver biopsies was
performed at baseline and 1-year follow-up. Results: Selleckchem Doxorubicin Pro-C3 plasma levels were significantly higher in HCV patients than in healthy controls (p < 0.001). HCV patients with F4 had significantly higher plasma Pro-C3 levels compared to patients with F3 (25.5 vs 17.4 ng/ml, 47% increase, p<0.05) and patients with F2 (25.5 vs 15.8 ng/ml, 61% increase, p<0.05). The diagnostic value for plasma Pro-C3 when separating controls from HCV patients was significant (AUC=0.83, p<0.0001) as well as for comparing mild fibrosis (F2/F3) to moderate fibrosis (F4) (AUC=0.72, p=0.0003). Patients with disease progression after 52 weeks (as determined by a higher Ishak score) had a higher plasma Pro-C3 level compared to patients who did not progress (21.4 vs 16.9 ng/mL; p<0.05). No differences were observed for plasma C3M. For Y-27632 cost evaluation of prognostic value the patients were further divided; 0: no change in Ishak stage; 1: increase of one Ishak stage; and 2: patients with an increase of two Ishak stages. There was an overall difference in plasma Pro-C3 (p=0.008) and plasma C3M (p=0.041) between the three groups. Mean plasma Pro-C3 was significantly
elevated in group 1 compared to group 0 (20.2 vs. 16.9 ng/ml, p<0.05), and plasma C3M was significantly higher in group 2 (23.4 ng/ml) compared to group 1 (17.1 ng/ml) and group 0 (18.4 ng/ml) (p<0.05 for both). The odds ratio for progression in the upper quartile of Pro-C3 was 3.4 (p=0.02). Conclusion: We assessed
type III collagen turnover in two different ways, formation and degradation. Only formation provided significant MCE公司 clinical value. Pro-C3 differentiated mild from moderate disease and was able to identify those patients in most need of treatment consequent to fibrosis progression. Disclosures: Sanne S. Veidal – Employment: Nordic Bioscience Mette J. Nielsen – Grant/Research Support: Nordic Bioscience A/S Morten A. Karsdal – Stock Shareholder: Nordic Bioscience Diana J. Leeming – Employment: Nordic Bioscience Zachary D. Goodman – Grant/Research Support: Gilead Sciences, Fibrogen, Galectin Therapeutics, Merck, Vertex Stephen D. Gardner – Employment: GlaxoSmithKline, GlaxoSmithKline, Glaxo-SmithKline, GlaxoSmithKline Robert Hamatake – Employment: GlaxoSmithKline; Stock Shareholder: GlaxoSmithKline Keyur Patel – Consulting: Benitec, Santaris; Speaking and Teaching: Gilead Sciences, Vertex Background: Hedgehog (Hh) pathway is innately active in liver development, and its further activation in response to injury stimulates biliary dysmorphogenesis in Biliary Atresia. Reactive ductules along the limiting plate are pivotal players in biliary fibrogenesis: they produce Hh ligand and are Hh-responsive, producing osteopontin (OPN), a profibrogenic cytokine.