PubMedCrossRef 35. Cappellaro C, Mrsa V, Tanner W: New potential cell wall glucanases of Saccharomyces cerevisiae and their Eltanexor in vivo involvement in mating. J Bacteriol 1998,180(19):5030–5037.PubMed 36. Rowe JD, Harbertson JF, Osborne JP, Freitag M, Lim J, Bakalinsky AT: Systematic
identification of yeast proteins extracted into model wine during aging on the yeast lees. J Agr Food Chem 2010,58(4):2337–2346.CrossRef 37. Penttilä ME, Suihko ML, Lehtinen U, Nikkola M, Knowles JKC: Construction of brewer’s yeasts secreting fungal endo-β-glucanase. Curr Genet 1987,12(6):413–420.CrossRef 38. Yang SL, Liu ZS, Chi SZ, He S, Meng QW, Liu CC, Lin Y, He GQ: Production of beer with a genetically engineered strain of S. cerevisiae with modified beta glucanase expression.
J I Brewing 2009,115(4):361–367.CrossRef 39. Gil JV, Manzanares P, Genoves S, Valles S, Gonzalez-Candelas L: Over-production of the major exoglucanase of Saccharomyces cereviside leads to an increase in the aroma of wine. Int J Food Microbiol 2005,103(1):57–68.PubMedCrossRef Competing interests The authors declare that they have no competing interest. Authors’ contributions TSB conducted all experiments and analyzed results. SJ contributed to analysis of proteome data and editing the manuscript. NA and TSB conceived the study and Bafilomycin A1 in vitro participated in its design, coordination, and draft of the manuscript. All authors have read and approved the manuscript.”
“Background Microorganisms, because of their phenomenal biodiversity, are a rich natural resource of many biologically active compounds such as proteins, CDK inhibition polyunsaturated fatty acids, pigments and polysaccharides [1, 2]. Metabolites produced by microorganisms often display high biological activities and their potential health benefits make them valuable ingredients in nutraceuticals, cosmetics
and the food industry [3, 4]. Moreover, investigations related to the search for new bioactive compounds from industrially important microbial strains are of continued importance because of the high potential economic value of these metabolites [5, 6]. Demand for carotenoid Axenfeld syndrome (CT) pigments has been growing annually at a rate of 3.1% and is a market predicted to reach at least US$ 1.17 billion in value by 2012 as consumers continue to look for natural alternatives. Among them, canthaxanthin (CX) is used extensively in the food, fishery, cosmetic, and pharmaceutical industries [7, 8]. D. natronolimnaea is one of the most important sources for the microbial production of CX from a commercial and industrial point of view [9, 10]. To meet the growing demand of CX, a cost effective scaling-up of the industrial process is imperative [11]. In conventional methodology, nutritional factors and others necessary for growth of the microorganism are optimized by changing one at a time while keeping all others constant. [12].
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