Statistical evaluations were performed as either a t-test or a Ma

Statistical evaluations were performed as either a t-test or a Mann–Whitney test using the GraphPad InStat version 3 program (San Diego, CA, USA). All the long-term cultured MS target cells express B cell markers on their surfaces, and as Rituximab® is an anti-B cell antibody, a combination of target cells and this antibody comprises a possible control system for ADCC, assessed as effector cell granularity expressed as CD107a expression. Three different target cell cultures, MS 1533, MS 1874 and MS 1946, were tested with effector cells from a total of 10 different donors. As seen in Table 1,

all target cells express sufficient amounts of B cell epitopes for the antibody to elicit CD107a expression on the effector cells. Results are given both with and without Rituximab®; the latter are Talazoparib concentration to be considered as NK cell activity. There is no difference in the relative number of CD56+ cells, and the CD107a expression is at similar levels for the NK activity, whereas ADCC activity with Rituximab® as the active antibody is increased significantly for all 10 effector

cell donors. The ADCC activity against each of the three different target cells also differs with Rituximab® as the RGFP966 research buy active antibody. CD56+ NK cells can be subdivided into two populations based on the relative expression of the surface marker CD56. These subsets, CD56bright and CD56dim, differ in their activity. CD56bright cells are a minor constituent of the NK population in PBMCs; they are Thymidylate synthase active cytokine producers but are only weakly cytotoxic before activation, whereas the CD56dim cells are the cytotoxic killers [12, 13]. As shown in Fig. 2, analyses of the distribution of CD56bright and CD56dim cells in the effector cell donors show certain variability in the relative proportions of the weakly cytotoxic CD56bright cells to CD56dim cells, which may have implications for the cytotoxic potential of the effector cells. A panel of polyclonal rabbit antibodies has been raised against selected HERV

epitopes. In Fig. 3 we illustrate the antibody reactivity by showing examples of HERV epitope expression and reactivity of anti-HERV H/F Gag- and anti-HERV-H antibodies on target cells. Figure 4 illustrates effector cell reactivity against target cells/anti-HERV antibodies, shown as flow cytometric profiles of induced changes in CD107a levels. Tables 2 and 3 summarize data for all antibodies, examples of effector cells and target cells, with high CD107a expression in CD56+ cells when antibodies against HERV-H/F Gag and HERV-H Env H1 were added to the target cells. A somewhat lower reactivity was observed with anti-HERV-H Env H2, whereas activity was negligible for the remaining anti-sera in the panel. The antibodies were tested against target cell cultures and effector cells as performed in the assays with Rituximab®. A similar reactivity pattern was seen against the target cells.

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