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Anti-Cdc2 antibody (PSTAIRE; Sigma Chemical) was used as loading control. Northern blot analysis Aliquots of the cultures were recovered at different times, total RNA preparations obtained and resolved through 1.5% agarose-formaldehyde gels, and hybridizations were performed as previously described [35]. … Continue reading →

Marco Camera is a specialist in Infectious Disease working at the IRCCS AOU San Martino-IST, Genoa Giovanni Orengo was medical director of San Martino-IST Hospital until 2011 and then director of hospital hygiene to date. His main goal was to … Continue reading →

Phys Rev B 2007, 75:140513.CrossRef 7. Anzai H, Ino A, Kamo T, Fujita T, Arita M, Namatame H, Taniguchi M, Fujimori A, Shen ZX, Ishikado M, Uchida S: Energy-dependent enhancement of the electron-coupling spectrum of the underdoped Bi 2 Sr … Continue reading →

Plant Mol Biol 2006, 60:717–27.PubMedCrossRef 9. Cavalieri D, Casalone E, Bendoni B, Fia G, Polsinelli M, Barberio C: Trifluoroleucine resistance and regulation of α-isopropylmalate synthase in Saccharomyces cerevisiae. Molec & Gen Genet 1999, 261:152–160.CrossRef 10. Frothingham R, Meeker-O’Connell WA: Genetic … Continue reading →

Steroid binding proteins have been described for various yeasts [42]. Many studies have predicted the existence of a progesterone receptor in the membrane of filamentous fungi such as Rhizopus nigricans[27–30] but the molecular basis of steroid signalling in fungi remains … Continue reading →

​cdc.​gov/​botulism/​botulism.​htm. The Caspase activity current gold-standard assay, the mouse protection bioassay, is impractical in situations needing high-throughput analysis of multiple samples possibly at multiple geographical locations. In 2003 the National Institute of Allergy and Infectious Disease (NIAID) issued recommendations for … Continue reading →

5 km), end of first lap (23.2 km), time to top of second climb (35.7 km) and finish (46.4 km). Throughout the trials, HR and Tre were recorded every 2 min, while self-reports of perception of effort [28], thermal sensation … Continue reading →

The proliferation:senescence balance is an important determinant of tumour progression, dormancy or regression. If the DN:DP ratio estimates this, it could have prognostic value. Although progenitor isolation using markers will never recapitulate the complexity of these plastic and diverse cellular … Continue reading →

[52]. Briefly, overnight cultures of S. epidermidis strains grown in TSB medium were diluted 1:200 and inoculated into wells of polystyrene microtiter plates (200 μl per well) and incubated at 37 °C for 24 h. After incubation, the wells were … Continue reading →

Int J Radiat Biol 2000, 76: 1297–1303.CrossRefPubMed 8. Courdi A, Brassart N, Herault J, Chauvel P: The depth-dependent radiation response of human melanoma cells exposed to 65 MeV protons. Br J Radiol 1994, 67: 800–804.CrossRefPubMed 9. Chiquet C, Grange JD, … Continue reading →