Human melanoma was not stimulated by 10 U/ml LPS (the activity was identical to that of the PBS control). Its migration was decreased by 31% (p = 0.0423) Nutlin-3a in vivo by T4 compared with PBS. A significant difference between PBS and HAP1 was not observed (28%, p = 0.0859) (Fig. 3). Expanded analysis of the effect of LPS (dose gradient) showed no significant or marked trend in the human melanoma response (Fig. 4). Figure 3 The effect of T4 and HAP1 bacteriophages on Hs294T human melanoma migration on fibronectin. The insert: the 8-μm 0.3-cm2 membrane was covered with fibronectin. Hs294T melanoma cells were applied at 1 × 105 cells per insert in DMEM.
The final concentrations of the bacteriophage preparations were 1.5–2.5 × 109 pfu/ml and 10 U/ml of residual LPS. The LPS control was also 10 U/ml (which equals 0.25 ng/ml). The concentration of the attracting agent, FBS, in the lower section of the migration chamber was 7.3–7.5%. Migration was carried out for 1 h 20 min at 37°C in CO2. The cells were stained and counted under light microscopy on the whole membrane. The mean number of cells per membrane (bars) and SD (lines) are presented. Figure 4 The effect of LPS on Hs294T human melanoma migration on fibronectin. The insert: the 8-μm 0.3-cm2
membrane was covered with fibronectin. Hs294T melanoma cells were applied at 1 × 105 cells per insert in DMEM. LPS was applied as a dose gradient (10 U/ml equals 0.25 ng/ml). The concentration of the attracting VX-680 supplier agent FBS in the lower section of the migration chamber was 7.3–7.5%. Migration was carried out for 1 h 20 min at 37°C in CO2. The cells were stained and counted under light microscopy on the whole membrane. The mean number of cells per membrane (bars) and SD (lines) are presented. Migration of human and mouse melanoma on www.selleckchem.com/products/crenolanib-cp-868596.html Matrigel matrix Matrigel matrix is a reconstituted basement membrane with a wider range of components, including stimulating and regulating factors and various proteins. It allows more complex and multiple interactions of cells during their motility and more
complete analysis of the migration process. The overall migration activity of B16 melanoma was poor and the Liothyronine Sodium results were strongly dispersed. Therefore the assay did not show a significant inhibition of B16 migration by T4 and HAP1 (Fig. 5). The LPS concentration gradient did not reveal any significant trend towards stimulation or inhibition related to the dose series, although the test was made with two complementary sets of doses. The dispersion of the results was also remarkable, which strongly hindered their analysis (Figs. 6 and 7). Figure 5 The effect of T4 and HAP1 bacteriophages on B16 mouse melanoma migration on matrigel matrix. The insert: the 8-μm 0.3-cm2 membrane was covered with matrigel (approx. 7 μg/cm2). B16 melanoma cells were applied at 4 × 105 cells per insert in DMEM. The final concentrations of the bacteriophage preparations were 1.5–2.
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