FL: follicle lumen. Porcine thyrocytes also showed strong APN activity at the apical pole of the cell (Figure 1e). In addition to thyrocytes,
also endothelial cells weakly expressed APN activity. In the other species studied, APN activity was restricted to endothelial cells in the peritumoral stroma (Figure 1f). Morphology, iodide uptake and protease activities in cultured thyrocytes In human thyrocytes, only DPP II but no activities for APN and DPP IV were detected, Selleckchem GDC 0068 suggesting that the isolation from the tissue did not cause prominent changes in the pattern of protease activities. To determine whether isolated cultured porcine thyrocytes also behaved similarly to thyrocytes in intact tissue, these cells were physiologically
characterized. Porcine thyrocytes formed functional follicles with characteristic thyrocyte morphology and with a stable preserved polarity in the CP673451 purchase presence learn more of TSH (right-side-right follicles, Figure 2a). These follicles showed microvilli at the apical surface and tight junctions between the cells, but no basement membrane formed at the basal pole of the cells. Upon stimulation with TSH, iodide uptake was increased by a factor of 6.8 relative to unstimulated controls (Figure 2b). This uptake was inhibited by 1mM perchlorate. Despite being an inhibitor of iodine organification, not of iodide uptake, thiamazole also significantly decreased iodide-uptake. Figure 2 Physiological behaviour of cultured porcine thyrocytes according to ultrastructure, iodide uptake and protease activity detected by synthetic substrate (red). a: Porcine thyrocytes form follicles with formation of apical microvilli and intercellular tight junctions when stimulated with 1.3 mU/ml TSH for 30h. b: Upon stimulation with TSH, iodide uptake of thyrocytes is increased 6.8 times compared to unstimulated cells (mean ± SEM is shown). TSH-induced Amisulpride iodide uptake is inhibited
by 1 mM perchlorate and significantly reduced upon exposure to TSH + 2 mM thiamazole (p < 0.05). c: Upon stimulation with TSH for 30h, DPP II activity is seen in all cells, whereas activity of APN at the plasma membrane in seen only in thyrocytes integrated in follicles but not in isolated cells (d, arrowhead). N: nucleus, FL: follicular lumen. Activities for all enzymes detected in intact tissues were also demonstrated in primary cultures of porcine thyrocytes when cultured in the presence of TSH. Intracellular localization of DPP II was seen in all cells (Figure 2c), but only thyrocytes integrated into follicles showed localization of APN at the plasma membrane (Figure 2d). Compared to APN, DPP IV activity was very weak. When cultured in the absence of TSH in porcine thyrocytes only DPP II could be detected (data not shown), whereas the activities of APN and DPP IV were below the detection threshold. In human thyrocytes, only DPP II activity, but not APN and DPP IV was detected.
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