This supports the notion that TIP60 might play an important role

This supports the notion that TIP60 might play an important role during Salmonella infection. This increase is SseF-independent, as similar increase was also observed when infected with an sseF mutant Salmonella strain and TIP60 was not concentrated at the vacuoles (data not shown). SseF was not detected in infected cells possibly due to the low amounts translocated during Salmonella infections. Selleckchem OICR-9429 Figure 3 TIP60 is up regulated upon Salmonella infection. HeLa cells were infected with wild-type Salmonella for the indicated time intervals. Infected cell lysates were subjected

to SDS-PAGE followed by Western blot using anti-TIP60 antibody (upper panel). Actin levels in the same samples were also determined as a control (lower panel). TIP60 is required for efficient intracellular Salmonella replication Previous studies have shown that SseF is required for efficient intracellular

Salmonella replication in macrophages [10]. Since TIP60 acetyltransferase interacts with SseF, TIP60 might be required for efficient intracellular Salmonella replication. To test this, we used siRNA to down-regulate the endogenous level of TIP60. Macrophages were transfected with a plasmid expressing TIP60 siRNA or a control vector expressing the SIS3 in vitro scrambled siRNA. As shown in Fig. 4, TIP60 siRNA effectively suppressed the endogenous TIP60 expression, while the control siRNA did not. Transfected macrophages were infected with wild-type S. typhimurium or the sseF mutant strains. As shown in Fig. 4, down-regulation of TIP60 leads to less efficient Montelukast Sodium Salmonella replication comparable to the level of sseF mutant strain [10]. There was not significant replication change in cells expressing the scrambled siRNA. These data support our notion that TIP60 is required for efficient intracellular Salmonella replication in macrophages. Figure 4 TIP60 is required for efficient Salmonella replication. Transfected macrophages

were infected with wild-type S. typhimurium or the sseF mutant strains at an MOI of 10. Extracellular bacteria were removed by washing and gentamicin treatment. At 2 and 24 h after bacterial invasion, cells were lysed, and the number of intracellular bacteria was enumerated. The data shown were obtained from three independent experiments with standard errors. The effect of TIP60 knockdown is verified by Western blot using the anti-TIP60 antibodies. Actin was used a control. Discussion We do not know yet the molecular mechanism of how SseF and TIP60 interaction affects the SCV and intracellular Salmonella replication. Ideally, a mutant SseF lacking the TIP60-binding domain can be used to PU-H71 mw assess the requirement for SseF-TIP60 interaction for its function, however such a mutant is defective in secretion and thus not translocated, making it impossible to assess its effect during infection.

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