2002) An impact site with non-viable populations on both sides o

2002). An impact site with non-viable populations on both sides of the road may be appropriate as well, but only if the combined amount of habitat on both sides of the road is sufficient for a viable overall population in the mitigated situation (Fig. 3c). Fig. 3 Schematic overview of consequences for population viability when populations of different size, above or below the threshold of a minimum viable population (MVP), are merged due to road mitigation measures. Viable populations are depicted in black, non-viable populations in grey. Populations in which a significant

effect of road mitigation on population viability is expected, here in situation B and C, should preferably be selected as research sites The size of a mitigation site, in terms of road length, may vary. Preferably, the mitigation site is delineated where the studied road effect no longer occurs. These boundaries typically occur buy OSI-027 where suitable habitat ceases. Hence, if more than one target species is studied at one mitigation site, the size of that site, in terms of road length, may differ for each species as habitat preferences differ among species. mTOR inhibitor The size of mitigation sites should not be based

on the length over which wildlife fences are planned—as they may only be planned for limited sections of the road. Limiting measurements to only fenced road sections may mean that the conclusions drawn about the effectiveness of the road mitigation measures may be overly positive (Fig. 4). Fig. 4 Example of how the size of a mitigation site, i.e., road length where measurements are carried out, affects conclusions about crossing structure effectiveness. The studied road effect is the reduction of between-population movement. The green area symbolizes suitable habitat for the studied species. Red areas are non-suitable habitat, e.g., urban areas. Road construction (II) has decreased the number of movements by 50 %, compared to pre-road Digestive enzyme conditions (I). If only the mitigated road stretch (C–D) is

included in the evaluation (III), the conclusion would be that the crossing structure is 100 % effective, as the number of movements across the road pre-road construction and post-mitigation are equal (n = 4). However, if the whole road stretch (A–B) is included in the evaluation, the conclusion would be that the crossing structure is only 70 % effective, as the crossing structure does not provide a solution for all potential movements across the road. Because the aim of the road mitigation was to fully prevent the barrier effect of the road between A and B, a delineation of the mitigation site between C and D will overestimate crossing structure effectiveness. Finally, the situation is shown where the full road length is fenced in (IV). In this case the effectiveness of the road mitigation measures is 40 %, illustrating that road mitigation, if not properly implemented, i.e.

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