A number of factors may influence the accuracy Wortmannin DNA-PK of EALs, such as the size of the apical foramen [25], the type and size of the measuring file [26], the irrigation solution used, and the electroconductivity of the pulp [27]. In the present study, two root canal sealers and three solvents were used, and we found no significant difference between the Tri Auto ZX ARL measurements and the TL in the presence of the different solvents in the root canal. There were significant differences between the Tri Auto ZX EL mode and the TL in the presence of solvents. Although it has been reported that EALs are reliable in the presence of different irrigants [28], the effects of root canal sealer solvents on EAL accuracy have not been evaluated before. Thus, we cannot compare the results of the present study with those studies.
However, in one similar study, Uzun et al. [7] compared two different combined devices (TCM Endo V and Tri Auto ZX) for removing gutta-percha and sealer from filled root canals with chloroform solvent and reported that the ARL function of both devices must be used with caution when removing gutta-percha root fillings.The size of the apical foramen [29] and the instrument used [30] have been reported to be important parameters for EAL accuracy. In the present study, we did not attempt to standardise the apical size of the samples, because it was not possible to find apically uniform-sized extracted teeth. Uniform-sized apical foramens can be generated by widening the roots progressively using bigger instruments, but with this technique, the apical anatomy is changed and the apically enlarged samples may not mimic real clinical conditions.
In the present study, we chose similar-sized teeth and used the same size instruments for all samples to increases the reliability of our results.Previous studies have reported that the orthograde retreatment technique should include additional enlargement of root canals beyond the initial canal preparation [2]. Thus, during retreatment procedures the MAF must be one or more sizes larger than the MAF used in the first preparations, as suggested by Ta?demir et al. [4]. In the present study, we used an F2 file for first preparation and an F3 file of the ProTaper system for repreparation of the root canals. The differences in ARL and EL measurements in the present study in the Resosolv and Endosolv groups may be attributable to the 0.
5 level choices for the apical limit because previous studies reported that EALs are more accurate when the APEX level is chosen as the apical limit [6, 21]. Also, the electrical conductivity of the dissolving agents may be responsible for the shorter lengths and poorer accuracy in the Resosolv and Endosolv E groups. However, we have no reasonable explanation of the differing accuracies between the same solvent sample and control groups (between Brefeldin_A Resosolv subgroups A2 and C3 and between Endosolv E subgroups B2 and C2).
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