Altough human errors cannot be completely abolished, continuous medical training and strict adherence to regulations should reduce the incidence to a minimum. New technologies are needed which will hopefully decrease the incidence of retained foreign bodies. An electronic article surveillance system which uses a tagged surgical sponge that can be identified electronically can be developed. Bar codes can be
applied to all sponges, and with the use of a bar code scanner the sponges can be counted on the back table. Key Word(s): 1. Gossypiboma; 2. Malabsorbtion; 3. Intestine; Presenting Author: JIXIANG ZHANG Additional Authors: MING TIAN Corresponding Author: GS1101 JIXIANG ZHANG Affiliations: The Second Affiliated Hospital of Nanchang University Objective: Genic regulation plays an important role in inhibiting the induction and EX 527 supplier growth of carcinomas. xeroderma pigmentosum D (XPD) plays an important role in nucleotide excision repair (NER) because of involvement in single-stranded DNA-dependent ATPase and DNA helicase activities. To observe the inhibitory effects of XPD on the growth of hepatoma cells and on the expressions of proto-oncogene Ets-1; also to examine
whether XPD down-regulates Ets-1 gene via P53. Methods: The human hepatoma cells (HepG2) were transfected with XPD expression vector constructed by our laboratory, subsequently incubated with Pifithrih-α (P53 inhibitor). The expression levels of XPD, P53, phosphp-P53(ser-15) and Ets-1 were detected by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. The cell viability was examined by MTT, and the cell cycle and apoptosis rate were detected by flow cytometry. Results: Compared learn more with the blank and control group, the cells transfected with recombinant plasmid XPD
showed high expression levels of XPD, P53 and phosphp-P53 (ser-15), but low expression level of Ets-1. XPD suppressed the viability of HepG2 and accelerated the apoptosis. Nevertheless, the aforementioned effects of XPD were partly abolished by the inhibition of P53. Conclusion: XPD may inhibit the growth of hepatoma cells, down-regulated the expression of Ets-1 through the P53 pathway. Key Word(s): 1. XPD; 2. Hepatoma cells; 3. P53; 4. Ets-1; Table 2 Companison of proteins expressions of XPD, P53, Ets-1 in six groups group XPD P53 p-P53 Ets-1 1 0.350 ± 0.076 0.440 ± 0.120 0.581 ± 0.236 1.327 ± 0.135 2 0.410 ± 0.031 0.355 ± 0.092 0.499 ± 0.139 1.282 ± 0.052 3 0.380 ± 0.068 0.402 ± 0.128 0.435 ± 0.157 1.196 ± 0.122 4 0.850 ± 0.193 1.001 ± 0.215 1.032 ± 0.280 0.609 ± 0.033 5 0.843 ± 0.295 0998 ± 0.273 0.377 ± 0.092 1.387 ± 0.334 6 0.362 ± 0.026 0.425 ± 0.091 0.463 ± 0.269 1.164 ± 0.172 F 7.864 10.173 3.955 8.062 P (1) : (3) 0.811 0.782 0.408 0.372 (2) : (3) 0.807 0.739 0.714 0.556 (1) : (4) 0.002 0.001 0.021 <0.001 (1) : (5) 0.002 0.002 0.253 0.678 (4) : (5) 0.952 0.981 0.002 <0.001 (4) : (6) 0.002 0.001 0.006 0.
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