In those cases where the analysed tissue had a high content of reducing sugars, this resulted in strong staining of the background. This interfering staining of the background was avoided by prior incubation with sodium borohydride. The specificity of the devised method was demonstrated in a parallel localization study using a specific antibody towards prunasin hydrolase.”
“The aim of this study was to investigate for the first time nesfatin-1-like immunoreactive (LI) cells in the mucosal layer of the canine digestive tract. Distribution of nesfatin-1 – LI cell bodies and the co-localization of nesfatin-1 with somatostatin (SOM), cocaine-and amphetamine-regulated Danusertib transcript peptide
(CART), protein gene product 9.5 (PGP 9.5) and vasoactive intestinal peptide (VIP) were studied by immunohistochemistry in the selected parts of the canine digestive tract. Evaluation of the nesfatin 1-LI cells number has been based on the counting of cell bodies per observation field. Emricasan in vitro Nesfatin-1-LI cells amounted to 0.87 +/- 0.06, 1.06 +/- 0.02, 0.85 +/- 0.11 and 0.56 +/- 0.13 in gastric fundus, duodenum, jejunum and descending
colon, respectively. The co-localization of nesfatin-1 with the other substances studied was not observed. Functions of nesfatin-1 – LI cells in the canine digestive tract are unknown. (C) 2012 Elsevier Ltd. All rights reserved.”
“This work is focused on the removal of Ni(II) from aqueous solutions by sorption onto newly developed magnetite-loaded calcium alginate particles. The uptake of Ni(II) by these magnetite particles, with their mean geometrical diameter 84 and 508 pm, is best described by the Freundlich isotherm and the constants K(F) and 1/n were found to be 3.491 mg g(-1), 0.731 and 0.793 mg g(-1) and 0.907, respectively. The mean sorption energy, as determined by Dubinin-Radushkevich isotherm for 508- and 84-mu m sized particles was evaluated to be 8.9 and 8.0 kJ mol(-1), respectively, Selleckchem A 1155463 thus, suggesting the ion-exchange mechanism for uptake process. Of the various kinetic models
proposed, the kinetic Ni(II)-uptake data were best interpreted by “”Simple Elovich”" and “”Power function”" as suggested by their higher regression values. The almost linear nature of plots of log(% sorption) versus log(time) was indicative of intraparticle diffusion. The values of intraparticle diffusion coefficients K(jd) were found to be 63.49 x 10(-2) and 94.35 x 10(-2) mg l(-1) min(0.5). The intraparticle diffusion was also confirmed by Bangham equation. Finally, various thermodynamic parameters were evaluated. The negative Delta G degrees indicated spontaneous nature of uptake process while positive Delta H degrees value suggested exothermic nature of the sorption process. (C) 2009 Wiley Periodicals, Inc.
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