Similar results were obtained when we electroporated the vMGE of chicken brain slices with control or Slit2-expressing plasmids, and monitored the migration of corridor cells by implanting a crystal of DiI in the LGE (ncontrol = 7, nSlit2 = 7; Figures 6D–6F). Finally, we showed that Slit2 has a direct repulsive activity in corridor cell migration within a 275 μm radius, by confronting in vitro mouse corridor cell explants to control or Slit2-expressing COS cells ( Figures 6G–6J; ncontrol = 20, nSlit2 = 21). Thus, Slit2 exerts a short-range repulsive activity in the migration of both chicken and mouse corridor cells. Because Slit2 is
differentially expressed in mouse and chicken embryos, we wondered whether variations of Slit2 expression might have an impact on the distribution of corridor cells in vivo. To this aim we performed in ovo electroporation of GFP control and Slit2-expression http://www.selleckchem.com/products/gsk1120212-jtp-74057.html plasmids in the ventral telencephalon of E3 chicken embryos, and examined corridor cell distribution using Islet1 immunohistochemistry at E6 ( Figures 6K–6N). We checked that we were accurately targeting the ventral telencephalon by examining GFP expression not only at E6, Bosutinib but also 20 hr post-electroporation, because the MGE and POA generate cells that colonize the entire ventral telencephalon (data not shown) ( Cobos et al., 2001). Although
GFP expression had no effect on the corridor (n = 16/16), we found that Slit2 ectopic expression induced a mild change in the distribution of corridor cells, resulting in an overall visible modification of the corridor shape in the absence of other morphological defects (n = 13/13) ( Figures 6M and 6N). Although these experiments are intrinsically variable, we reproducibly observed until a change in the corridor shape of Slit2-electroporated embryos that resulted in a significant decrease of
both the medial extension and the thickness of the corridor compared to the control situation ( Figure 6L). Taken together, our results indicate that in ovo modifications of Slit2 expression domain modify the distribution of the corridor, suggesting that Slit2 repulsive activity participates to this process in vivo. To further determine the role of Slit2 in the migration of corridor cells in vivo, we examined Slit2 mutant mice ( Plump et al., 2002). We first demonstrated using slice cultures that the repulsive activity of the vMGE&POA in corridor cell migration is abolished or drastically reduced in the absence of Slit2 function ( Figure S3). To investigate whether Slit2 inactivation affects the formation of the corridor, we performed a detailed in situ hybridization study in E13–E14.5 wild-type and mutant embryos, using a panel of molecular markers of corridor cells (Ebf1, Meis2, Nrg1), striatal neurons (Ebf1, Meis2, Foxp2) ( Ferland et al., 2003), and of the surrounding MGE and POA-derived structures (Lhx6, Nkx2.1, Shh). In agreement with previous studies ( Bagri et al., 2002 and Marin et al.