05) (Fig. 2). We also examined the MMP-inhibitor marimastat in the invasion assay to investigate a possible relationship
between invasion capacity and MMP expression. Marimastat inhibit the both cells invasion significantly (SaOS-2: 55 ± 6%, U2OS: 36 ± 4%, p < 0.05) (Fig. 3). Figure 2 Risedronate impedes the invasiveness of SaOS-2 and U2OS cells (A and B). A 10-well chemotaxis chamber was used to measure the effect of risedronate on invasiveness. A Matrigel-coated membrane was inserted between the upper and lower chambers, and stained using a Hemacolor rapid staining kit. Stained areas represented numbers of migrating cells. The numbers in the panels show the concentration of risedronate added. Images are representative of three independent experiments. Bars (C) represent cells number (expressed as percentages of controls) of each image ± standard deviation. CYT387 Figure 3 MMP-inhibitor Marimastat (50 μg/mg) impedes the invasiveness of SaOS-2 and U2OS cells. Three different experiments with each cell line were performed. Bars represent the cell numbers (expressed as percentages of controls) of each image ± standard deviation. Abbreviations: C: control; M: Marimastat. Risedronate INCB28060 cell line reduced MMP-2 and MMP-9 activities
in SaOS-2 and U2OS cells Since MMP-2 and MMP-9 play a critical role in tumor cell invasiveness, we examined the effect of risedronate on the enzyme activities of MMP-2 and MMP-9. Semaxanib Accordingly, gelatin zymography was conducted using conditioned media harvested from risedronate treated SaOS-2 and U2OS cells. The gelatinolytic activities of both MMP-2 and MMP-9 were found to be reduced in both cell lines after treatment with increasing concentrations of risedronate, which suggested that the reductions in cell invasion by risedronate is a consequence of reductions in the activities of MMP-2 and MMP-9 (p < 0.05) (Fig. 4). Figure 4 Risedronate
inhibited the gelatinolytic activities of MMP-2 and MMP-9. (A) Conditioned media harvested from SaOS-2 and U2OS cells treated for 48 h with the indicated concentrations of risedronate were analyzed by gelatin zymography. The white bands represent MMP-mediated gelatin digestion. The image is representative of three independent experiments. MMPs activities (expressed as percentages of controls) are shown in B (n = 3). Numbers in boxes represent the concentration of risedronate (in μM) added MAPK inhibitor to cells. Bars represent the MMPs activities (expressed as percentages of controls) of each band ± standard deviation. Risedronate reduced MMP-2 and MMP-9 protein levels in both cell lines To investigate whether risedronate inhibits the expressions of MMP-2 and MMP-9, SaOS-2 and U2OS cells were treated with risedronate and MMP-2 and MMP-9 protein levels were determined by Western blotting. As shown in Fig. 5, Western blotting revealed that risedronate inhibit MMP-2 and MMP-9 protein levels (p < 0.05). Figure 5 Risedronate reduced the expressions of MMP-2 and MMP-9 proteins in SaOS-2 and U2OS cells.
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