1) for AFP-L3 ≥35% and 3.5 (1.9-6.7) for DCP ≥7.5 ng/mL; p=0.004, 0.003, 0.002, 0.0003 and <0.0001, respectively. The HR (95%CI) increased to 5.2 (2.3-12.0) for patients with both AFP ≥250 ng/mL and DCP ≥7.5 ng/mL, p<0.0001. Among patients with tumors within the Milan criteria, the HRs (95%CI) were 3.1 (1.3-7.5) for AFP ≥250 ng/mL, 4.3 (1.8-10.1) for DCP ≥7.5 ng/mL, and
4.5 (1.9-10.6) for AFP-L3 ≥35%; p=0.01, 0.0008, 0.0005, respectively. The HR (95%CI) for tumors outside the Milan criteria increased from 2.6 (1.4-4.7) to 8.6 (3.0-24.6), and 7.2 (2.8-18.1) when combined with AFP ≥250 ng/mL, and DCP ≥7.5 ng/mL respectively (p<0.0001 for both). The concordance index (95%CI) of MLN8237 the Milan criteria increased from 0.63 (0.56-0.70) to 0.68 (0.60-0.76), 0.70 (0.62-0.78) and 0.70 (0.62-0.78) when combined with AFP, DCP and AFP-L3%, respectively,
suggesting that combining the biomarkers with the Milan criteria was more predictive of recurrence than the Milan criteria alone. Conclusions: HCC biomarkers significantly improved the performance of the Milan criteria in predicting HCC recurrence after LT. Our findings could potentially improve the organ allocation algorithm for LT. Disclosures: Shinji Satomura – Board Membership: Wako Life Sciences, Inc Lewis R. Roberts – Grant/Research Support: Bristol Myers Squibb, ARIAD Pharmaceuticals, BTG, Wako Diagnostics, Inova Diagnostics, Gilead Sciences The GS 1101 following people have nothing to disclose: Roongruedee Chaiteerakij, Xiaodan Zhang, Benyam D. Addissie, Essa A. Mohamed, William S. Harmsen, J Paul Theobald, Brian
E. Peters, Joseph Balsanek, Melissa M. Ward, Nasra H. Giama, Catherine D. Moser, Abdul M. Oseini, Naoki Umeda, Denise M. Harnois, Michael Charlton, Hiroyuki Yamada, Alicia check details Algeciras-Schimnich, Melissa R. Snyder, Terry M. Therneau Injury of donor bile ducts may lead to the development of non-anastomotic biliary strictures (NAS) after liver transplantation. Peribiliary glands (PBG) provide a niche of progenitor cells that contribute to regeneration of biliary epithelium of large bile ducts. It is unknown whether PBG injury plays a role in the development of NAS after transplantation. Aim of this study was a) to determine the degree of PBG injury in donor livers, b) to assess whether PBG injury is a risk factor for the development of (NAS), and c) to identify risk factors for PBG injury in donor livers. In 128 liver transplant procedures, biopsies were taken from the extrahepatic bile duct (EHBD) and injury was assessed using a systematic histological grading system. Histological injury was correlated with the occurrence of NAS. Donor characteristics and surgical variables were correlated with PBG injury, using uni- and multivariable regression analysis. . In another10 donor livers that were declined for transplantation, proximal extension of bile duct injury was assessed by obtaining biopsies from the EHBD and the intrahepatic sectoral and segmental ducts.