, 1987 and Bento and Miniti, 1989), but full functional recovery is seldom achieved. Nerve repair requires a complex interaction among a scaffold for axonal growth guidance, supportive cells such as Schwann cells, growth factors, and extracellular matrix (Da-Silva et al., 1985, Costa et al., 2006 and Costa et al., 2009a). The combination of axonal scaffolds and transplanted cells provides adequate support for neural regeneration, and has been investigated as a strategy to overreach the limitations of surgical repair (Evans et al., 2002, Cheng and Chen, 2002, Udina et al., 2004 and Rodrigues et al., 2012).
In particular, the polyglycolic acid tube (PGAt), composed of absorbable material, has been established as an appropriate conduit for nerve grafting, and has been approved by the Food and find more Drug click here Administration (FDA, USA) for use in the clinical setting (Mackinnon and Dellon, 1986, Da-Silva et al., 1987, Mackinnon and Dellon, 1990, Weber et al., 2000, Costa et al., 2006, Costa et al., 2009b, Schlosshauer et al., 2006 and Nectow
et al., 2011). Isolated and cultured Schwann or stem cells have been employed in the surgical repair of the peripheral nerve (Dezawa et al., 2001, Cuevas et al., 2002, Evans et al., 2002, Fansa and Keilhoff, 2004, Udina et al., 2004, McKenzie et al., 2006, Chen et al., 2007, Lavdas et al., 2008, Ishikawa et al., 2009, Wang et al., 2009, Wakao et al., 2010, Wei et al., 2010, Ladak et al., 2011, Wang et al., 2011, Rodrigues et al., 2012 and Salomone et al., 2013). Schwann-like cells have been reported to differentiate in vitro from bone marrow stroma mesenchymal stem cells (BMSC)
primarily cultured from rat femurs ( Dezawa et al., 2001 and Chen et al., 2007). Schwann-like cells experimentally employed in peripheral nerve repair have improved myelination ( Dezawa et al., 2001, Glutamate dehydrogenase Cuevas et al., 2002, Chen et al., 2007, Ishikawa et al., 2009 and Wang et al., 2011). Although there are limited data on the association of PGAt and genetically modified BMSC-derived Schwann-like cells in the repair of the facial nerve ( Shi et al., 2009), a thorough, objective analysis on the functional nerve recovery and of in vivo cell survival is lacking. Our approach in the current study has been to employ the gold-standard nerve repair procedure, nerve autografting, combined to bone marrow mesenchymal stem cells seeded in purified basement membrane as a secondary scaffold, used to fill the lumen of PGAt. Our aims were to compare the facial nerve functional and morphological outcomes, and to evaluate the presence and phenotype of the exogenous cells in the autografted nerve, six weeks after implantation. The use of five different animal groups allowed for progressive addition of each component to be tested.
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