1b) Using multiple regression analysis, we evaluated independent

1b). Using multiple regression analysis, we evaluated independent effects of genetic and non-genetic factors on the development of thyroid autoantibodies. The reference categories for the analysis were CT60 CTLA-4 genotype, age, family history of AITD and cigarette smoking. In the case of thyroid peroxidase antibodies, CAL-101 nmr we confirmed a significant contribution of CT60 CTLA-4 genotype (P < 0·007) and younger age (P < 0·05), while family history and cigarette smoking did not prove to have any effect. In thyroglobulin antibodies, no contribution of either genotype or non-genetic factors

was confirmed. The genotyping in the group of 75 PPT patients revealed the AA genotype in 17 (22·7%) patients, the AG genotype in 36 (48%) and the GG genotype in 22 (29·3%) patients, showing no deviation from HWE (χ2 0·096, P = 0·757). As presented in Table 2, the patients with different genotypes did not differ in age, number of pregnancies, family history of AITD and smoking status. However, females with the G-allele carrying genotypes presented significantly more often with positive values of thyroid peroxidase antibodies (P < 0·04), while

the proportion of thyroglobulin antibody-positive patients did not differ significantly between the three genotypes. Similarly, more patients with the G-allele carrying genotypes had at least one type of thyroid autoantibody elevated compared

to the AA genotype (P < 0·04) (Table 2). Furthermore, the median value of thyroid peroxidase antibodies was Y-27632 manufacturer significantly lower in the AA genotype compared to the AG and GG genotypes (median, 12, 130 and 423 U/ml, respectively, P < 0·006) (Fig. 2a). In contrast to thyroid peroxidase antibodies, the median values of thyroglobulin antibodies did not differ significantly between the three genotypes (Fig. 2b). For the evaluation of thyroid autoantibody Baf-A1 nmr development with multiple regression analysis, the reference categories were CT60 CTLA-4 genotype, age, number of pregnancies, family history of AITD and cigarette smoking. For thyroid peroxidase antibodies, we established a significant contribution of CT60 CTLA-4 genotype (P < 0·04), while the effect of other factors was not confirmed. In thyroglobulin antibodies, no significant contribution of genetic or non-genetic factors was found. In PPT patients, 41 (54·7%) were hyperthyroid at presentation, while hypothyroidism was established in 34 (45·3%) patients. As presented in Table 3, the median value of thyroid peroxidase antibodies was significantly higher in the hypothyroid form of disease (P < 0·0001). Similarly, the median value of thyroglobulin antibodies was higher, although the difference was statistically insignificant.

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