, 2006). Nodulation assays on Glycyrrhiza uralensis with wild-type Selleckchem XL184 and quorum-sensing-deficient mutant strains of M. tianshanense showed that mrtI and mrtR mutants were unable to develop nodules on legume roots. This may have been due to poor bacterial attachment by the mutants, because the mrtI strain showed a 60% reduction
of root hair attachment efficiency (Zheng et al., 2006). Exopolysaccharides were recently shown to be involved in biofilm formation in M. tianshanense (Wang et al., 2008). Sequence analysis of nonmucoid strains showed that mutations were located in two gene clusters: the first is similar to pssNOPT of Rhizobium leguminosarum bv. viciae (Young et al., 2006), and the second is similar to the exo5 gene in R. leguminosarum bv. trifolii (Laus et al., 2004). All these genes are conserved among rhizobia and are involved in exopolysaccharide polymerization and translocation (Skorupska et al., 2006). The mtpABCDE genes responsible for exopolysaccharide production in M. tianshanense are regulated by the two-component histidine kinase regulatory
system MtpS–MtpR (Wang et al., 2008). The exopolysaccharide-deficient strains mtpC, mtpR, and mtpE failed to nodulate G. uralensis and formed a biofilm with smaller biomass compared with the wild type in the borosilicate attachment assay, suggesting that exopolysaccharides are essential for biofilm formation (Wang et al., 2008). Quorum-sensing mechanisms control numerous functions in rhizobia, Neratinib concentration including exopolysaccharide production (Marketon et al., 2003; Hoang et al., 2004; Glenn et al., 2007), motility and nitrogen fixation (Hoang Isotretinoin et al., 2004, 2008), and nodulation (Cubo et al., 1992; Rodelas et al., 1999; Daniels et al., 2002; Hoang et al., 2004), all of which are related to symbiosis. The studies cited in this section show clearly that Mesorhizobium is one of the genera of bacteria in which quorum sensing plays an important role in biofilm formation, attachment, colonization, and nodulation of legumes.
Since biofilm formation was first reported in Sinorhizobium meliloti (Fujishige et al., 2005), soil microbiologists have been interested in rhizobial regulatory systems in this species, and conditions for analyzing its ability to produce biofilms. Biofilm formation is clearly an important feature of this species’ symbiotic ability, and its resistance to adverse environmental conditions. Biofilm production on abiotic surfaces (glass or plastic) has been used as a model for characterization of bacterial aggregation and attachment (O’Toole & Kolter, 1998b). Use of this approach in S. meliloti has helped clarify the roles of nutritional and environmental conditions (Rinaudi et al., 2006), exopolysaccharides and flagella (Fujishige et al., 2006), ExoR with the ExoS–ChvI two-component system (Wells et al., 2007), nod genes (Fujishige et al.
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