24 However, few studies have reported yield of high molecular weight DNA from formalin fixed tissues.13 Thus, the effect of formalin on DNA as a probable cause of negative results in PCR assay is ruled out. On the other hand, several studies
mostly from low incidence areas used extracted DNA from non fixed, frozen tissues as well as cell lines of ESCC (not exposed to formalin) and similarly showed negative results in PCR or southern Inhibitors,research,lifescience,medical blot hybridization techniques.18, 25 Moreover, using the same materials, primers and instruments, a PCR assay of cases with uterine cervical 5-Fluoracil cost carcinoma in this center successfully revealed the presence of common and type specific HPV DNA sequences in many of the cases. So, negative findings in our study can’t be attributable to a sensitivity problem in the PCR assay. Conclusion The findings of the present study indicate that there is no Inhibitors,research,lifescience,medical association between HPV infection and development of esophageal squamous cell carcinoma in the cases evaluated. Acknowledgment The authors would like
to thank Mr. Abdul-Mohammad Pezeshki for his great Inhibitors,research,lifescience,medical cooperation and technical support. This study was supported by a grant (number; 81-1528) from Vice Chancellor for Research, Shiraz University of Medical Sciences. Conflict of interest: None declared.
Dear Editor, Endotracheal intubation (ETI) is a simple, rapid, safe and non-surgical technique for airway management. It maintains airway patency, protects the lungs from aspiration, and permits leak-free ventilation during mechanical ventilation. The anesthesiologists’ knowledge, technical Inhibitors,research,lifescience,medical skills and crisis management capabilities play an important role
in controlling the occurrence of unwanted complications during airway management.1 In anesthesiology, leaking cuff is one of the problems occurring after correct endotracheal intubation. It usually occurs when the cuff is damaged by contact with teeth, nasal bone or Magill forceps during mechanical ventilation Inhibitors,research,lifescience,medical in which the tracheal tube should be removed and replaced. In patients with impossible manipulation of the neck, injuries to the cervical vertebrae of their neck, or difficult intubation, it is necessary to use a fiberoptic bronchoscope for reintubation most of the times. Direct laryngoscope with Macintosh blade for intubation Terminal deoxynucleotidyl transferase is the first selection in cases of difficult intubation.2 Using laryngoscope produces reflex sympathetic stimulation, and subsequently increases heart rate, blood pressure, intracranial pressure, myocardial ischemia, and cardiac arrhythmia. Complications of airway problems often result in morbidity and mortality in anesthesia.1 Inadequate cuff seal is common and leads to hypoventilation during mechanical ventilation and aspiration of gastric contents. The most common cause of reintubation during anesthesia in the operating room is upper airway obstruction and hypoventilation.
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