4��g/mL solutions (A2/A0.4) was calculated by the mean values of peak areas in a different ratio of acetone and methanol. The results (Figure 2) showed that A2/A0.4 for 1:4 and 1:0 solutions were 8.2 and 6.4, respectively, while others so were close to 5.0, equal to the concentration proportion of BDE-209. This could explain the inaccuracy of correlation coefficient (r2) when acetone made up more proportion. With the limited solubility of BDE-209 in methanol, it is necessary that the mixed solutions can totally dissolve the BDE-209 in environmental samples. Thus, a ratio of 1:1 (acetone and methanol) was selected for optimal combination, with a r2 of 0.9992 (Figure 3), for BDE-209 concentration range 0.2 to 8��g/mL. Figure 2Different ratios of methanol and acetone on peak area of BDE-209 (blue line represents the peak area ratio of 2��g/mL and 0.
4��g/mL solutions (A2/A0.4) at various amount of acetone).Figure 3Standard curve of BDE-209.3.2. Verification of Method FeasibilityIn order to investigate the performance of the proposed method, the soil samples from grassland were analyzed under the above conditions. The soils were analyzed in four parallel samples. The results (Table 1) showed good repeatability with a RSD of 1.0%. Due to the high concentration of BDE-209 that was detected in the simulated soil, it indicated that 1:1 of acetone and methanol can completely satisfy the analysis of environmental soil samples.Table 1Concentration of BDE-209 in different sites. As the feasibility of the method in the analysis of contaminated simulated soils, this research was further concentrated on verification of method feasibility on real environmental soil samples (Table 1).
The RSD was 11.4%, 8.9%, and 5.9% for QBS, GBS, and GPF, respectively. E-waste contaminated soils are usually more complex which contain a large number of PBDEs, PCBs, PAHs, and heavy metals, leading to measurement difficulties. Results of QBS, GBS, and GPF indicated that the developed method was feasible. The factor of impurity interference should be considered during analysis. White precipitate was observed in chromatography bottle when it was reconstituted with acetone and methanol. There was no report on this phenomenon when it was reconstituted with hexane [32, 33]. The pretreatment was a crucial step for accurate quantification of BDE-209; Entinostat more soil samples extracted with solvents will inevitably lead to an increase in impurities. The polarity of the solvent system was enhanced when methanol was added to the simple system of acetone, which leads to the white solid precipitation.
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