5 against Listeria monocytogenes, Yersinia enterocolitica and Aeromonas hydrophilla suggesting a synergic interaction. Only for Pseudomonas fluorescens FIC index was 0.75 purposing additive effect. Application of the essential oils alone (MIC) or in mixture (1/4 MIC + 1/4 MIC or 1/4 MIC + 1/2 MIC) in vegetable broth caused significant decrease (p<0.05) in bacterial count over 24 h. Mixture of essential oils reduced (p<0.05) the inocula of all bacteria in vegetable broth and in experimentally inoculated fresh-cut vegetables. Similar efficacy was found to reduce the autochthonous
microflora in vegetables. Sensory evaluation of vegetables sanitized with essential oils JNJ-26481585 ic50 revealed that the scores of the most evaluated attributes fell between like slightly and neither like nor dislike.
The combination of essential oils at sub-inhibitory concentrations could mean an interesting approach to sanitize minimally processed vegetables. (C) 2011 Elsevier Ltd. All rights STA-9090 reserved.”
“To ensure the implementation of genetically modified organism (GMO)-labeling regulations, an event-specific detection method was developed based on the junction sequence of an exogenous integrant in the transgenic carnation variety Moonlite. The 5′-transgene integration sequence was isolated by thermal asymmetric interlaced PCR. Based upon the 5′-transgene integration sequence, the event-specific primers and TaqMan probe were designed to amplify the fragments, which spanned the exogenous DNA and carnation genomic DNA. Qualitative and quantitative PCR assays were developed employing the designed primers and probe. The detection limit of the qualitative PCR assay was 0.05% for Moonlite in 100 ng total carnation genomic DNA, corresponding to about 79 copies of the carnation haploid genome; the limit of detection and quantification of the quantitative
PCR assay were estimated to be 38 and 190 copies of haploid carnation genomic DNA, respectively. Carnation samples with different contents of genetically modified components were quantified and the bias between the observed and true values of three samples were lower than the acceptance criterion SNS-032 (<25%) of the GMO detection method. These results indicated that these event-specific methods would be useful for the identification and quantification of the GMO carnation Moonlite.”
“This study aims to assess fatigue aspects in Moroccan patients with ankylosing spondylitis (AS), and its relationships with disease-specific variables of activity and severity. A cross-sectional study included patients with ankylosing spondylitis according to New York Classification criteria for the diagnosis of AS. To assess fatigue, the first item of Bath Ankylosing Spondylitis Disease Activity index (BASDAI) and the Multidimensional assessment of fatigue were used (MAF).
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