The expression
level of DKK-1, an inhibitor of canonical WNT signaling, was decreased in Cal27cis, a sub-cell line of Cal27, and was obtained by treating Cal27 with increasing concentrations of cisplatin. Overexpression of DKK-1 in both Cal27 and Cal27cis resulted in increased sensitivity to cisplatin, suggesting DKK-1 and the WNT signaling pathway as a marker and target for cisplatin chemosensitivity. In human glioma cells, a previous study showed that transfection of DKK-1into human glioma cell line U87MG causes the cells more sensitive to cisplatin and alkylating agent [15]. Our current study revealed that the expression of bax and caspase-3 increased, whereas the expression of bcl-2 decreased in the SHG44 -DDK-1 this website cells, further confirming the pro-apoptosis function of DKK-1. We speculate that the function of DKK-1 may be tissue or cell type specific. Another possibility is that mutations of DKK-1 could be PLX3397 the causes of different functions of DKK-1. A screening of 73 brain tumors, however, revealed that no obvious mutations of DKK-1 were found in these brain tumors [21]. More studies, especially direct comparison of DKK-1 in different cell types at the same condition, are needed in order to better understand
the P005091 mw complex functions of DKK-1 in relation to cancer development. Acknowledgements This work was partially supported by major issues Foundation of health department in Jiangsu province (K 200508). This manuscript has been edited and proofread by Medjaden Bioscience Limited. References 1. Glinka A, Wu W, Delius H, Monaghan AP, Blumenstock C, Niehrs C: Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction. Nature 1998, 391: 357–362.PubMedCrossRef 2. Krupnik VE, Sharp JD, Jiang C, RG7420 molecular weight Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA: Functional and structural diversity of the human Dickkopf gene family. Gene 1999, 238:
301–313.PubMedCrossRef 3. Yamabuki T, Takano A, Hayama S, Ishikawa N, Kato T, Miyamoto M, Ito T, Ito H, Miyagi Y, Nakayama H, Fujita M, Hosokawa M, Tsuchiya E, Kohno N, Kondo S, Nakamura Y, Daigo Y: Dickkopf-1 as a novel serologic and prognostic biomarker for lung and esophageal carcinomas. Cancer Res 2007, 67: 2517–2525.PubMedCrossRef 4. González-Sancho JM, Aguilera O, García JM, Pendás-Franco N, Peña C, Cal S, García de Herreros A, Bonilla F, Muñoz A: The Wnt antagonist DICKKOPF-1 gene is a downstream target of beta- catenin/TCF and is downregulated in human colon cancer. Oncogene 2005, 24: 1098–1103.PubMedCrossRef 5. Wirths O, Waha A, Weggen S, Schirmacher P, Kühne T, Goodyer CG, Albrecht S, Von Schweinitz D, Pietsch T: Overexpression of human DICKKOPF-1, an antagonist of wingless/WNT signaling, in human hepatoblastoma and Wilms’tumor. Lab Invest 2003, 83: 429–434.PubMed 6.
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