In our experiment, we used a 408-nm excitation wavelength laser. Optical sections were averaged three times to reduce noise. RNase A@C-dots for in
vivo fluorescence imaging Male 4-week-old athymic nude mice were purchased SB-715992 chemical structure from Shanghai Slac Laboratory Animal Co. Ltd (Shanghai, China). All experiments that involve animal use were performed in compliance with the relevant laws and institutional guidelines. All animal experiments were approved by the Institutional Animal Care and Use Committee of Shanghai Jiao Tong University (No. SYXK2007-0025). For the establishment of the tumor model, MGC-803 cells were resuspended in PBS, and 2 × 106 cells per site were subcutaneously injected. The tumor nodules had reached a volume of 0.1 to 0.3 cm3 approximately 3 weeks post-injection. For in vivo fluorescence tumor imaging experiments, 100 μl (5 mg/ml) RNase A@C-dot aqueous solution was intratumorally injected into the MGC-803 tumor-bearing mice. Time-course fluorescent images (excitation, 500/20 nm; emission, 600/30 nm; integration time, 5 s) were acquired on a Bruker In-Vivo F PRO imaging system (Bruker, Billerica, MA, USA). Results and
discussion Characterization and properties of RNase A@C-dots TEM images of the as-prepared RNase A@C-dots that were trapped in the dialysis membrane (MW cutoff 1,000) are shown in Figure 1a; the size of the RNase A@C-dots varies mainly within 25 to 45 nm with relatively irregular
morphologies. High-resolution TEM image (Figure 1b, the zoomed-in Entinostat image of the area within the circle in Figure 1a) selleck products clearly shows that the particles are actually formed by encapsulating several C-dots within the RNase A film, so we can call them clusters. The clusters can also extremely easily disperse in pure water. In Figure 1c, the average size of C-dot that dispersed out of the dialysis membrane is about 4 nm (Figure 1f) in diameter with nice spherical morphologies (Figure 1d), and the dispersions are also excellent. Lattice spacing of approximately Carbohydrate 0.23 nm clearly displayed in the high-resolution TEM image (Figure 1d) indicates the (100) facet of graphite [30]. Figure 1 TEM and HR-TEM images, XRD pattern, and size distribution of RNase A@C-dots. (a) TEM image of the as-prepared RNase A@C-dots inside the dialysis membrane after dialyzing against pure water. One typical RNase A@C-dot cluster is labeled with a black circle. (b) High-resolution TEM (HR-TEM) image of one focused area within the black circle. (c) TEM image of the C-dots outside the dialysis membrane. (d) HR-TEM image of one single C-dot. (e) XRD pattern of RNase A@C-dots. (f) Size distribution of C-dots. We can reasonably conclude that during the reaction process accelerated by microwave heating, RNase A capped the different numbers of C-dots that cause the different sizes of particles.
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