A differential response in larval feeding patterns was observed on the plants engineered with novel glucosinolates. Larvae click here fed on plants overexpressing 4-hydroxybenzyl glucosinolate and isopropyl glucosinolate showed little response. Larvae fed on 35S:CYP79A2
plants engineered to overexpress benzyl glucosinolates, however, showed reduced larval and pupal weights. Upon herbivory a high expression of JA signalling gene LOX2 was observed on the 35S:CYP79A2 plants compared to the PR1a and VSP2 expression. To confirm the role of benzyl isothiocyanate (BITC), a degradation product of benzyl glucosinolate overexpressing plants, in the retarded larval growth we used Virus Induced Gene Silencing (VIGS) approach to silence LOX2 expression in the 35S:CYP79A2 plants. S. littoralis larvae fed on LOX2 silenced 35S:CYP79A2 plants exhibited a retarded larval growth thus indicating that BITC played a pivotal role in anti-herbivory and not only the JA signalling pathway. (C) 2011 Elsevier Masson SAS. All rights reserved.”
“Background: Patients with human immunodeficiency virus (HIV) infection on protease inhibitors (PIs) have a heightened risk of arterial SBE-β-CD mouse thrombosis but little is known about treatment-naive patients. Methods/Results: Prospective study
from South Africa comparing thrombotic profiles of HIV-positive and -negative patients with acute coronary syndrome (ACS). A total of 30 treatment-naive HIV-positive patients with ACS were compared to 30 HIV-negative patients with ACS. Patients with HIV were younger; and besides smoking (73% vs 33%) and low high-density lipoprotein (HDL; 0.8 +/- 0.3 vs 1.1 +/- 0.4), they had fewer risk factors. Thrombophilia was more common in HIV-positive patients
with lower protein C (PC; 82 +/- 22 vs 108 +/- 20) and higher factor VIII levels (201 +/- 87 vs 136 +/- 45). Patients PLX3397 solubility dmso with HIV had higher frequencies of anticardiolipin (aCL; 47% vs 10%) and antiprothrombin antibodies (87% vs 21%). Conclusion: Treatment-naive HIV-positive patients with ACS are younger, with fewer traditional risk factors but a greater degree of thrombophilia compared with HIV-negative patients.”
“Cadmium (Cd), a non-essential metal, is a kind of toxic heavy metal to life, which can accumulate in rice tissues including seeds, thus posing a risk to human health through food chain. To investigate the molecular mechanisms of rice response to Cd exposure, suppression subtractive hybridization and mirror orientation selection were used to compare gene expression profiles in seedling roots of Cd-exposed and control (unexposed) rice plants (Oryza sativa L, Nipponbare). Approximately 1700 positive clones, with insertions ranging from 250 to 1300 bp, were identified through reverse cDNA microarray analysis. Gene expression was further confirmed by real time RT-PCR.
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