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“Agrobacterium vitis strain Z-VAD-FMK mouse E26 is a promising biocontrol agent of grapevine crown gall, an economically important disease of grape worldwide. In this report, we developed a Plating-PCR method that allows specific detection and quantification of E26 by combining classical microbiological
techniques with molecular tools. Random amplified polymorphic DNA fingerprints were used to differentiate E26 from other A. vitis strains. A differentially amplified fragment from E26 was sequenced and characterized as a sequence characterized amplified region (SCAR) marker. Two primer pairs were then designed and evaluated for their specificity against E26. One of the two SCAR primer pairs, 740F/R, was further selected for specific detection of strain E26. A plating assay coupled to PCR with the SCAR primers 740F/R allowed LDK378 in vitro the assessment of population dynamics of E26 in non-sterile grape rhizosphere soil under controlled conditions. “
“During the year 2008 to 2009, a new disease of stem canker was noticed in most red-fleshed
dragon fruit (Hylocereus polyrhizus) plantations in Malaysia. The symptoms observed were small circular sunken orange spot, black pycnidia and rotted stem. This study was conducted to determine the occurrence of the stem canker on H. polyrhizus in Malaysia, subsequently to isolate, identify and characterize the fungal pathogen based on morphology selleck inhibitor and molecular characteristics and pathogenicity test. From the surveyed 20 plantations in Malaysia, stem canker was detected in all the plantations. A total of 40 isolates of Scytalidium-like fungus were isolated and identified as Neoscytalidium dimidiatum based on morphological characteristics and ITS region sequences, which showed 99% similarity to N. dimidiatum (FJ648577). From the phylogenetic analysis using maximum-likelihood tree, isolates of N. dimidiatum from stem canker of H. polyrhizus were grouped together and did
not show any sequence variation. From pathogenicity test, all 40 isolates of N. dimidiatum were pathogenic causing stem canker on H. polyrhizus. To our knowledge, this is the first report of stem canker of H. polyrhizus caused by N. dimidiatum in Malaysia. “
“Corynespora cassiicola (Berk. & Curt.) Wei is an important phytopathogenic fungus, and different isolates show great diversity in their reproductive structures. Therefore, the aim of this study was to evaluate the potential of the API-ZYM® system as an auxiliary tool in the polyphasic approach of C. cassiicola identification. Five C. cassiicola isolates from different host plants and one Pseudocercospora griseola isolate were tested. A typical enzymatic pattern was obtained, with eight enzymes being produced by all five C. cassiicola isolates. An intraspecific differentiation was also found.
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