of cytosolic tyrosine kinases Src, the proteolytic metalloproteinase mediated, Gie S, heparin AZD6482 epidermal from growth factor binding its Preferences Shore transmembranespanning extracellular Ren HB EGF in the second stage the free HB EGF trans-activated EGF receptors in the cells self-closing and s in the usual way, the hot t is the RTK of the EGF receptor phosphorylation and internalization, the direct Src kinase, Ras and Raf dependent- ngigen ERK phosphorylation . It is unclear whether anything similar signaling pathways in astrocytes, which are of mature astrocytes in primary family members Rkulturen traffic again followed. However, the epidermal growth factor transforming a, HB EGF and amphiregulin, detected in brain tissue, and we found EGF, TGF, and HB EGF expression in astrocytes.
It is also not known whether the phosphorylation of ERK1 / 2 induces gene expression in astrocytes, and whether dexmedetomidine activates the phosphorylation of ERK1 / 2 in cultured neurons. In the present study we investigated the ways of the need during the events that tested activated to a ligand of the EGF receptor and that the EGF GDC-0941 receptor stimulation release, whether dexmedetomidine or conditioned medium caused dexmedetomidinetreated of astrocytes and transactivation of ERK1 / 2 in zerebell re granule neurons in primary rkultur.
Was for the first goal to test whether the transactivation by adrenergic stimulation, or St Tion a2 transduction by PTX, an inhibitor of the dissociation of the subunits of the protein bg gay or GF 109203X, an inhibitor of protein kinase C can be inhibited when Src kinase was from and / or after the liberation of the EGF receptor ligands in the study of the effect of PP1, a Src kinase inhibitor of both EGF and dexmedetomidine ERK1 / 2 phosphorylation, if involved in ERK1 / 2 phosphorylation by dexmedetomidine induced, but not by EGF may be inhibited by GM 6001, h depends an inhibitor of metalloproteinase Zn, the EGF receptor phosphorylation by dexmedetomidine by AG 1478, GM 6001 inhibited PP1 and GF 109203X, when increased hte phosphorylation of ERK1 / 2 was induced by dexmedetomidine exclusively Lich in the cytosol or p ERK1 / 2 was also entered the nucleus, and if early genes were induced by dexmedetomidine and whether the induction of AG 1478, or U0126, an inhibitor of the ERK are inhibited.
Materials and methods of animal cell cultures have been all work in accordance with the NIH guidelines for the care and use of animals in research and protocols from Locational animal ethics committee approved the China Medical University t. Prim rkulturen Of astrocytes, newborn Mice CD 1 of both sexes were as previously described with minor modifications. Ren Neopallia the hemisphere, Which corresponds approximately to the forebrain, were isolated from fa We aseptic vertebral dissociation of the tissue through nylon nets with a pore E of 80 and then 10 mm, filtered, diluted growing medium and planted in soil Your Falcon Primaria culture. The culture medium was Dulbecco’s medium initially glucose 7.5 mm, First with 20% horse serum and the cultures were incubated at 37 1C in a humidified atmosphere re incubated CO2/air. The culture medium was supplemented with fresh media Hnlicher composition on day 3 and subsequently replaced every 3 days 4th From the 3rd Day, the serum concentration reduced to a minimum
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