(D) Nuclear staining of Sox2 in normal bronchial epithelium cells, squamous metaplasia and squamous cell carcinomas. (E) Cytoplastic and nuclear staining of Msi2 in normal bronchial epithelium cells, squamous metaplasia and squamous cell
carcinomas. (F) Negative immunostaining signal of Nanog in normal lung, cytoplastic staining of Nanog in squamous metaplasia and squamous cell carcinomas. (G). Negative immunostaining signal of OCT4 in normal lung and tuberculosis, nuclear staining of OCT4 in small cell lung carcinomas. All images were taken at 400× magnification. In non-malignant lung tissues, CD133 was exclusively expressed in some, but not all, bronchial epithelium cells and bronchial selleck screening library smooth muscle cells (Figure 2C). CD133+ bronchial epithelium cells were found in 74% of non-malignant lung tissues while CD133+ bronchial smooth muscle cells were 70%. In lung cancer tissues, about 56% of tumor samples were diffusely positive, 8% focally MK-0518 supplier positive and 2% isolated positive for CD133 (Figure 2C). In non-malignant lung tissues, all bronchial epithelium and squamous metaplasia showed positive expression
of Sox2 (Figure 2D) and Msi2 (Figure 2E), the expression decreases in terminal bronchioles and was absent in alveolar epithelial. In lung cancer, the expression of Sox2 and Msi2 was 90% and 94% respectively, and more than 85% of tissues was diffusely positive for both of the markers (Figure 2D, E). In non-malignant lung tissues, only 2 cases of squamous
metaplasia Selleckchem MK 2206 4-Aminobutyrate aminotransferase in non-tumor adjacent lung tissues were positive for Nanog (Figure 2F), whereas, Nanog staining was detected in 36 of 50 (72%) cases of lung cancer, in which 29 cases were diffusely positive, 6 cases were focally positive and 1 case was isolated positive (Figure 2F). In all non-malignant lung tissues, no positivity for OCT4 was observed (Figure 2G). In lung cancer group, only one case of SCC and one case of SCLC were focally positive for OCT4 (Figure 2G). Potential value of the expression of stem-cell-associated markers as diagnostic markers Table 4 describes the specificity, accuracy and sensitivity of seven stem-cell-associated markers mRNA in bronchoscopic biopsies of lung cancer and non-cancer patients. The stem-cell-associated markers with the highest sensitivity for malignancy were CD44 (98.2%), Sox2 (98.2%) and Msi2 (96.4%), but their specificity were too low to be considered of no clinical significance. Nanog exhibited the highest specificity which was 66.7%, and its sensitivity was 63.4%. Table 4 The specificity, accuracy and sensitivity of seven stem-cell-associated markers mRNA in biopsy samples obtained from bronchoscopy Specificity, % Accuracy, % Sensitivity, % Bmi1 33.3 80.8 88.4 CD133 44.4 80 85.7 CD44 11.1 86.2 98.2 Sox2 16.7 86.9 98.2 Nanog 66.7 63.8 63.4 OCT4 61.2 82.3 85.7 Msi2 5.6 83.8 96.
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