foetus are distinct species ( Felleisen, 1998 and Tachezy et al., 2002). Trichomonads have a high endocytic Nintedanib in vitro activity as shown in previous studies where large particles, such as polystyrene microspheres (Benchimol et al., 1990), bacteria (Benchimol and De Souza, 1995) and yeast cells (Pereira-Neves and Benchimol, 2007), were ingested by these protists. The binding process is the first step to endocytosis. Thus, to address whether the different shapes of T. mobilensis presented distinct binding activity, adherence trials using latex beads were carried out. No differences
in the attachment of microspheres were found in all T. mobilensis shapes suggesting that the different forms of this parasite exhibited the same binding activity behavior. However, the binding capability of both T. foetus isolates was significantly higher than the binding capability of both T. mobilensis strains. This difference between the two species could be really greater than the difference between
individuals or strains within a species. However, to confirm this point, the binding capability of other strains of both species should be evaluated and compared. In higher eukaryotic cells, vesicular cell traffic ceases during mitosis and the endoplasmic reticulum and Golgi complex break down into small vesicles as the nuclear envelope does (Darnell et al., 1995). In contrast to this, the present study shows that both T. mobilensis and T. foetus maintain their adherence activities during all phases of the mitotic process. Similar observations were found during ingestion Z-VAD-FMK order of yeast cells by T. vaginalis ( Pereira-Neves and Benchimol, 2007). Different endocytic abilities have been reported for several trichomonas isolates
and a virulence correlation has been established for the trophozoitic forms (Juliano et al., 1991, Rendón-Maldonado et al., 1998 and Pereira-Neves and Benchimol, 2007). Here we demonstrate that T. foetus presented higher endocytic ability when compared with T. mobilensis. Therefore, we decided to assess the cytotoxicity of both species. T. foetus and T. mobilensis were co-incubated with host cells, such as caco-2 cells (a large-intestinal cell line). This experimental set up was chosen as an epithelial model for interaction studies because both parasites may be found in intestinal epithelium during in vivo infections ( Culberson Fossariinae et al., 1986 and Tolbert and Gookin, 2009). The MTT assay was carried out to compare the cytotoxicity of T. mobilensis and T. foetus after interaction with host cells. This method is frequently employed for the detection of cell viability following exposure to pathogenic microorganisms ( Ishiyama et al., 1996). MTT is a water soluble tetrazolium salt, which is converted to an insoluble purple formazan by the active enzyme, succinate dehydrogenase, within the mitochondria. After the reaction, the formazan product formed is impermeable to cell membranes. Therefore, it accumulates in healthy cells ( Mossmann, 1983).
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