Food allergy and atopic eczema during the first years of life have been considered risk factors for subsequent asthma and rhinitis caused by indoor and outdoor inhalant allergens [5, 9, 10]. However, allergy-like symptoms in childhood like wheezing and eczema need not have an atopic background they [11, 12]. Wheezing episodes in young children are often transient and associated with viral respiratory infections [13, 14]. Thus, better predictors of disease development and diagnostic markers for allergic disease are needed to give proper treatment and valuable advice, especially concerning environmental control to parents of young children [15]. The present study was carried out in order to evaluate the diagnostic performance of Phadiatop Infant, an in vitro test designed to detect allergen-specific IgE antibodies known to be relevant in the development of IgE-sensitisation in early childhood.
2. Patients and Methods 2.1. Patients The study was conducted retrospectively in consecutively included children, below the age of five years and admitted to BKL Voksentoppen, Rikshospitalet, Oslo, during a period of twelve months. The children were referred from paediatricians and other paediatric departments at hospitals in Norway and thus selected for more severe allergic symptoms. Frozen-serum samples from 122 children were analysed and clinical data documented in the patients’ records were compiled for the study. The study conformed to the principles of Helsinki’s declaration and was approved by the local Ethics Committee. 2.2. Skin Prick Test Skin Prick Test (SPT) was performed according to standard procedure at the hospital.
Glycerinated allergen extract (Soluprick ALK-Abello, Denmark and a standard lancet with 1mm tip and a shoulder (ALK-Abello, Denmark) was used. Histamine chloride 10 mg/mL was used as positive-control and as negative-control glycerol. The reaction was recorded as 3+ when the reaction was equal to the histamine control, 2+ when half the histamine control, and 4+ when double the histamine control. The panel included egg white, cow’s milk, cod fish, peanut, hazel nut, wheat, house dust mite, cat dander, birch, timothy, Cladosporium, and latex. 2.3. Total and Allergen-Specific IgE Antibodies Total and allergen-specific IgE antibodies were determined in ImmunoCAP 100 (Phadia AB, Sweden) at the hospital laboratory when appropriate according to clinical findings.
The instructions from the manufacturer were followed. The same allergen panel as for SPT was analysed. 2.4. ImmunoCAP Dacomitinib Phadiatop, fx5, and ImmunoCAP Phadiatop Infant Serum samples were sent to Phadia AB, Uppsala, Sweden, for determination of specific IgE antibodies using ImmunoCAP Phadiatop, fx5 (a food mixture of milk, egg white, fish, peanut, wheat, and soy bean), and ImmunoCAP Phadiatop Infant using ImmunoCAP100 (Phadia AB, Uppsala, Sweden). Results �� 0.35 kA/l were considered positive.
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