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“Paratuberculosis is a highly prevalent chronic mycobacterial infection of the small intestine of ruminants. It causes substantial economic losses at farm level, particularly in cattle [1]. Transmission of the causative organism Mycobacterium avium subspecies paratuberculosis (MAP) amongst ruminants occurs by excretion via feces into the environment, where it may survive for prolonged periods of time [2]. When the disease progresses towards the clinical stage of infection, MAP can also be present in milk [3]. As a result of the latter it may represent a food safety issue given
the possible association between MAP and human Crohn’s disease [4]. Currently, a vaccine to control paratuberculosis
Olaparib datasheet in cattle is not available, since the whole cell vaccine registered for use in sheep interferes with control programs against bovine tuberculosis. Individual MAP proteins as subunit vaccine candidates may overcome this interference. selleck chemical In bovine paratuberculosis [5] and [6], similar to other mycobacterial diseases such as tuberculosis and leprosy, heat shock proteins (Hsp) elicit strong cell mediated and antibody responses. Our previous studies indicated that immune responsiveness to recombinant MAP Hsp70 proteins in naturally infected animals was predominantly cell mediated [6] and [7]. Since protective immunity to intracellular mycobacterial pathogens is thought to be cell mediated [8], recombinant MAP Hsp70 protein was used as a subunit vaccine in cattle concomitant with experimental infection with MAP. It induced protection as indicated by significantly reduced bacterial shedding [9]. In addition, Liothyronine Sodium MAP Hsp70 subunit vaccination did not interfere with current diagnostic methods to diagnose bovine TB [10]. Surprisingly, and in strong contrast with our previous observations in field cases of bovine paratuberculosis, this immunization-challenge study showed limited cell mediated responses against MAP Hsp70 and
pronounced MAP Hsp70 specific antibody production in the vaccinated animals [9]. The contribution of antibodies to protection against mycobacterial infections is disputed by some (reviewed in [11] and [12]), and supported by others (reviewed in [13]). Most of the recent studies on serum therapy of M. tuberculosis (MTb) infection report protective effects of antibodies specific for polysaccharide bacterial cell wall antigens such as the polysaccharide lipoarabinomannan (reviewed in [14]). In mice, a monoclonal antibody (Ig A) directed against a small surface-expressed mycobacterial heat shock protein (the 16 kD α-crystallin homologue) protected against early infection of murine lungs with MTb [15].
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