However, this effect was most likely associated with a decreased bacterial burden since previous studies demonstrated elevated IL-6 from UV-A (340-450 nm)
exposed fibroblasts [53, 54] and minimal effects of UV-A (1 J/cm2) treated keratinocytes on IL-6 production [55]. Interestingly, attenuation of IL-6 after 405 nm treatment was only evident if 405 nm irradiation was applied promptly after infection; the effect was lost if applied 24 h post-infection. We believe that at this later time point, multiple chlamydial proteins were already secreted by type III secretory pathways into the host cytoplasm and interacted with pattern recognition receptors (PRRs) resulting in IL-6 production. Previously, we have identified CCL2 as a risk factor for trichiasis Ceritinib chemical structure [13], and therefore analyzed the effect of 405 nm irradiation on C. trachomatis induced CCL2 production. To our knowledge, our findings are the first to demonstrate elevated levels of CCL2 after C. trachomatis infection in HeLa cells. In vivo analysis has shown elevated mRNA levels of CCL2 at two days post-infection with C. trachomatis mouse pneumonitis (MoPn) strain [29]. Unlike IL-6, the use of 405 nm phototherapy on C. trachomatis infected
HeLa cells did not have a significant selleck effect on CCL2 production. More studies are needed to further understand the relationship between C. trachomatis infection and CCL2 production resulting in these inflammatory differences. Conclusions With increasing evidence to support persistent infections amongst a percentage
of chlamydial infections post-antibiotic treatment [18–21, 32–34], it is important to look for alternative treatments. In this study, we have provided the first in vitro evidence for anti-bacterial effects against an intracellular bacterium, C. trachomatis, using 405 nm irradiation administered by portable LEDs. The reduction in bacterial numbers and IL-6 concentrations, and the clinical safety of 405 nm below irradiation, supports further studies evaluating its use as a phototherapy against chlamydial infections within the conjunctival and reproductive tract mucosae. The ability of photo treatment to penetrate mucosal tissue layers was demonstrated within the gastric mucosa against Helicobacter pylori using 408 nm light [36]. Together, these data provide a plausible alternative treatment against chlamydial infections and expands the anti-bacterial properties of 405 nm irradiation to include intracellular bacteria. Methods Cell line and bacterial stock Human cervical adenocarcinoma cell line HeLa 229 (HeLa) and C. trachomatis serovar E were kindly provided by Dr. Deborah Dean (Children’s Hospital Oakland Research Institute, Oakland, CA) and were used following previous protocols [56, 57]. HeLa cells were cultured and maintained in minimal essential medium (MEM; Sigma Aldrich Corp., St.
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