In the Isabel juices, concentrations of Ca, Fe and Zn in samples with addition of seeds were not significantly different INK 128 mw from the control juice. However, the concentration of Mg was slightly increased in these juices. On the other hand, the concentrations of Ca and Zn were significantly different in the Bordo juice with seed concentration of 200 g/kg in comparison to the Bordo control juice. Regarding the quantified elements, the correlation between seed concentration
and the concentration of mineral elements was tested for all varietal juices. The linear effect of treatments was investigated for the elements that showed significant differences in concentration when compared to control juices. For the Concord juices, positive correlation (p < 0.01) LDK378 price was observed for Ca (r = 0.97), Mg (r = 0.80) and Mn (r = 0.77). In the Bordo juices, good correlations were verified for K (r = 0.93), Mg (r = 0.82), Ca (r = 0.85) and Zn (r = 0.84) (p < 0.01). Positive correlation
between seed addition and the concentrations of Na (r = 0.67) and Mg (r = 0.69) were observed in the Isabel juices. The isotopes measured in this study were 63Cu, 60Ni, 88Sr, 75As, 52Cr, 7Li, 138Ba and 27Al. The metal contamination in grape juice samples is presented in Fig. 3. Copper was the predominant element in all the varietal juices, with higher levels observed in the Isabel juices, as shown in Fig. 3(A). In these juices, the Cu concentrations ranged from 386.2 ± 20.1 to 421.6 ± 4.2 μg/L. The average Cu concentration in the Concord and Bordo juices ranged
from 209.7 ± 4.0 to 244.9 ± 8.3 μg/L and 206.2 ± 3.1 to 251.6 ± 6.1 μg/L, as presented in Fig. 3(B) and (C), respectively. Concentrations of Cu in the V. labrusca L. juices were found to be below the permitted limits for inorganic contaminants only in beverages, in accordance with the Brazilian resolution that establishes the limit of 10 mg/L ( ANVISA, 2005). Among all the varietal juices, the metal concentrations were found to range between 3.0 ± 1.2 and 7.6 ± 1.1 μg/L for Ni, 102.5 ± 1.1 and 155.4 ± 0.9 μg/L for Sr, 1.6 ± 0.2 and 3.5 ± 1.2 μg/L for As, 94.6 ± 2.2 and 124.4 ± 2.4 μg/L for Cr, 3.0 ± 0.7 and 8.8 ± 0.5 μg/L for Li, 79.1 ± 0.5 and 124.2 ± 0.9 μg/L for Ba, and 112.2 ± 3.4 and 211.5 ± 5.5 μg/L for Al. Concord juices showed higher concentration of Li in comparison to Bordo and Isabel juices. The Bordo juices showed the lowest concentrations of Ni and Al, whereas the concentrations of Cr, Sr and Ba were found to be similar among the varietal juices. The addition of grape seeds had no significant effect on the metal contamination in juice samples.
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