Louis, MO) in #5015, PMI Mouse diet (Harlan
www.selleckchem.com/products/otx015.html Teklad, Madison, WI) for 2 and 3 weeks before sacrifice. Controls received normal chow. All animal studies were approved by the University of Pennsylvania Institutional Animal Care and Use Committee. Six-micrometer sections were briefly postfixed to slides using methanol-free 4% formaldehyde/1× PBS and rinsed with 1× PBS. Antigen retrieval suitable for cryostat sections was performed as described.31 Sections were blocked with 1% bovine serum albumin in 0.1% Triton X-100/1× PBS and incubated with primary antibodies (Supporting Information Table 1) at 4°C overnight. Slides were incubated with the appropriate Cy3- (1:600) or Cy5-conjugated (1:400) secondary antibodies (Jackson ImmunoResearch, West Grove, PA) for 2 hours at room temperature then counterstained with DAPI. YFP was detected using a cross-reacting antibody against green fluorescent protein (GFP). Images were captured using
a Nikon E600 microscope (Nikon, Melville, NY) equipped with a QICAM CCD camera (QImaging, Burnaby, BC, Canada) and processed using iVision software (BioVision Technologies, Exton, PA). We assessed at least 10 portal tracts from each animal and confirmed results by repeating stains in nonsequential sections using representatives from each treatment group. See Supporting Information Methods for Sirius Red staining, primary cholangiocyte isolation and culture, soluble factor treatment, immunocytochemistry, cell imaging, and real-time polymerase chain reaction. MK0683 To carry out in vivo lineage tracing, we generated Alfp-Cre × Rosa26-YFP mice, in which YFP is constitutively expressed in all cells derived from precursors that express the hybrid albumin
promoter and alpha-fetoprotein (AFP) enhancer (hepatocytes, cholangiocytes, and their bipotential progenitors; Supporting Information Fig. 1A). Efficient recombination occurred in embryonic progenitors, resulting in YFP marking of greater than 98% of K19-, A6-, and HNF4α-positive cells in postnatal livers (Figs. 1, 2B; Supporting Information Fig. 1B).32 To assess whether primary cholangiocytes from our reporter strain are able to undergo EMT in vitro, we treated them with transforming growth factor-beta1 (TGF-β1) either alone or in combination with tumor Venetoclax necrosis factor-alpha (TNF-α), which has been reported to drive EMT by way of stabilization of Snail.33 Cells treated with TGF-β1 for 72 hours appeared to lose cell-cell contacts and developed a fibroblast-like morphology (Fig. 2A). A TGF-β receptor inhibitor abrogated this effect, whereas combined TGF-β1/TNF-α treatment enhanced the phenotype. TGF-β1 treatment, alone and combined with TNF-α, also resulted in intracellular relocalization of E-cadherin from cell membranes and increased expression of α-SMA (Fig. 2C,D). At 72 hours, combined TGF-β1/TNF-α treatment yielded rare cells possessing α-SMA stress fibers (Fig. 2C), a characteristic of activated myofibroblasts. These were not contaminating cells, as they expressed YFP (Fig.
Related posts:
- Studies were performed in mouse cholangiocytes isolated from norm
- These blots have been washed, incubated with HRP anti mouse IgG s
- Initial, we examined recruitment of STAT3 in to the mouse Mn SOD
- The literature reports induction of obesity by a high fat diet in
- The literature reports induction of obesity by a high fat diet in