p.i., and to a maximal level reached at late times in the infective cycle, 36 h.p.i. As a control, and Enzalutamide cell line as expected, we observed no difference in the levels of ERK1/2
during infection. Additionally, viral stimulation of JNK1/2-P was blocked in a dose-dependent manner [10, 20, 40 and 50 μM (Fig. 1C, lanes 4–7)] when VACV infection was performed in the continued presence of SP600125. Similar results were obtained with CPXV infection (data not shown). In order to investigate whether the Orthopoxvirus-stimulated JNK1/2-P was biological relevant to the virus, we performed multi-step viral growth curves (MOI = 10) in the presence or absence of SP600125. Cellular extracts were collected at 3, 6, 12, 24, 36 and 48 h.p.i and assayed for viral yield. We observed that the SP600125-mediated inhibition played a relevant role in both VACV and CPXV biology. A significant reduction in the viral titers (⩾1 log reduction) was observed when VACV (Fig. 2A) or CPXV (Fig. 2B) infections were carried out in the continued presence of SP600125. To verify that the inhibitory effect associated with SP600125 was not restricted to the A31 cells, BSC-40 were
infected with VACV or CPXV as described above. As shown in Fig. 2C and D, treatment with SP600125 resulted in a severe decrease in viral production (2–3 log reduction) thereby demonstrating that viral growth inhibition is not cell-type specific. Additionally, we investigated whether SP600125 was able to affect MVA replication. To that end, BHK-21 cells were infected with MVA as described above. Again, our results showed (Fig 2E) that the inhibitor caused a learn more significant decline in virus yield (nearly 3 log reduction); while a more mild decrease (1 log) in infectivity was noted with VACV and CPXV (2F and 2G). The variation in the levels of inhibition caused by SP600125 might be due to the viruses’ tropism within different species such as murine (A31 cells), monkey (BSC-40 cells) and hamster (BHK-21 cells). Arachidonate 15-lipoxygenase In order to investigate at what stage the progression of the viral cycle was affected by SP600125, BSC-40 cells were left untreated (Fig 3A, B and C) or were pretreated with the inhibitor (Fig 3D, E, F and G) and infected with
VACV at an MOI of 2. At 18 h.p.i, infected cells were harvested and examined by electron microscopy. While infected cells in the absence of inhibitor (panels A, B and C) contained the full spectrum of virion morphogenesis forms characterized by the identification of crescent, spherical, immature virions (IV), immature virions with nucleoids (IVN) and brick-shaped mature virions (IMV), cells pre-incubated with SP600125 (panels D, E, F and G) showed a severe impairment of morphogenesis progression. Large virosomes surrounded by crescents were repeatedly detected. IVs could be also observed, however IVNs or IMVs were rarely seen. Identical phenotype was also observed when cells were infected with CPXV in the presence of SP600125 (data not shown).
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