Pcat924 showed better efficiency
Bcl-2 inhibitor (more than 10-fold increase in AlX activity compared to Pcat300) under the optimized culture conditions. Induction of the catR promoter with 0.20% H2O2 and 1.5% CaCO3 in the culture medium, further increased expression of AlX 2.61- and 2.20-fold, respectively, clarifying its inducible nature. Specific induction or repression of the catR promoter provides the possibility for utilization of this promoter in heterologous protein production. Filamentous fungi have been used for decades as major producers in the pharmaceutical, food, and food processing industries because of their GRAS (‘generally recognized as safe’ in the terminology of the US Food and Drug Administration) status, and their ability to secrete large amounts of protein. Previous studies suggested that Aspergillus niger is an ideal host organism for production of recombinant proteins (Roberts et al., 1992; Tellez-Jurado et al., 2006; Karnaukhova et al., 2007; Zhang et al.,
2008). For the efficient Selleckchem Ruxolitinib production of the recombinant protein, strong promoter sequences are required. Various promoters of different categories have been reported from many filamentous fungi. Inducible promoters which are not affected by catabolite repression include endoxylanase (exl A) from Aspergillus awamori (Gouka et al., 1996) and TAKA amylase (amyA) from Aspergillus oryzae (Tsuchiya et al., 1992). Among the strongest inducible promoters regulated by carbon catabolite repression are the glucoamylase A promoter (glaA) of A. niger var. awamori (Ward Liothyronine Sodium et al., 1990) and the Trichoderma reesei cellobiohydrolase 1 (cbh1) promoter (Ilmen et al., 1996). A constitutive promoter used across fungal species is the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase gpdA (Punt et al., 1992). Till 2007, only the glucoamylase A promoter (glaA) from A. niger has been used for the expression of heterologous proteins. Recently, a new inducible promoter Psuc1 from
A. niger AB1.13 was characterized (Roth et al., 2007). To obtain a new, promising promoter for the expression of heterologous protein production, we targeted promoter of catR gene from A. niger because some strains of A. niger are efficient producers of catalase. It is anticipated that a high catalase producer might have a strong promoter and as such, there are no reports on the use of catR promoter in expression systems. Hence it is a legitimate target for cloning and exploitation. In this attempt, we developed the constructs and checked the expression of alkaline xylanase gene transcriptionally fused under the catR promoter from A. niger and also addressed the length and nature of the catR promoter. Aspergillus niger taken from the culture collection of IIIM, Jammu, was used throughout the study (Traeger et al., 1991). The strain of A. niger used in the study was maintained on potato dextrose agar (PDA).
Related posts:
- PIK-90 showed that FSH deficiency
- The outcomes showed that lapatinib only weakly inhibited growth, EMT and Akt/GSK
- Certainly, our review showed that, as opposed to wild-type p53, w
- However, their efficiency measured in vitro did not correlate wit
- Flp In INS one cell lines conditionally expressing HNF4 from a 5