The formation of lipid droplets in the cytoplasm, mineral nodules and cartilage extracellular matrix in the mDPSC culture after chemical defined conditions confirmed
the adipogenic, osteogenic and chondrogenic differentiation potential, respectively. Not all the cells in mDPSC cultures had the differentiation capability and, in fact, a uniform induced differentiation free of non-responsive cells is very difficult to achieve in mesenchymal stem cell cultures.35 Interestingly, some elongated cells spontaneously acquired a contractile capacity. In addition of the induced differentiation described in this study, in one isolate it was observed spontaneous differentiation in adipocyte lineage (data not shown). These data indicate the high selleck screening library plasticity of the mDPSC even in the absence of specific stimuli. Stem cells obtained from human or rat dental pulp also exhibit extensive capability of osteogenic, chondrogenic and adipogenic differentiation.6, 7 and 11 However, Balic and Mina34 demonstrated that cultures derived from pulps of unerupted and erupted mouse incisors were incapable of differentiating into adipocytes and chondrocytes. The authors
suggest that the differentiation in these cell types may be masked by the significant number of osteo/progenitor cells present in the culture which should be investigated in experiments aiming to evaluate the differentiation potential as in vivo transplantation assays. The time of culture, the cell Protein Tyrosine Kinase inhibitor passage or medium used are other factors that may have hampered the differentiation of the cell isolates obtained
by Balic and Mina. This study provides the description of stem cells obtained from mouse dental pulp, generating cell lines positive for GFP that can be used to track the fate of these cells when injected into different mouse models of disease. The data presented herein demonstrate that mDPSC comprise a morphologically heterogeneous population of cells that exhibit some phenotypic and functional features of both embryonic and mesenchymal stem cells, such as observed in the human dental pulp. The ability to expand and differentiate opens the futures possibilities Masitinib (AB1010) in the study of the cell therapies in animal models. Funding: This work was supported by CNPq, FAPESB, FINEP, and FIOCRUZ. Competing interests: There are no conflicts of interest. Ethical approval: All of the experimental were approved by the Animal Ethics Committee of the Gonçalo Moniz Research Center-FIOCRUZ, Salvador, Bahia. “
“Despite numerous investigations1, 2, 3, 4 and 5 the precise mechanisms involved in the formation and enlargement of jaw cysts have not been completely established. Cyst formation is believed to be related to the proliferation of epithelial remnants that are activated by the release of cytokines and growth factors.
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