The morphological changes caused by the rpoN mutation can be accompanied by the alteration of bacterial membrane and cell wall, and this website would possibly result in permeability changes. H2O2 is non-ionic and freely passes through membranes. Thus, the rpoN mutation may interfere with the permeability of H2O2 and confer resistance to H2O2; however, this possibility will be examined in future studies. Conclusions As a zoonotic JQEZ5 chemical structure foodborne pathogen, C. jejuni
encounters various environmental stresses during transmission and infection, such as changes in osmolarity, temperature and the high acidic pH in the stomach; only the bacteria that survive in these deleterious stresses can reach human hosts. Thus, the ability of C. jejuni in stress resistance can be Selleckchem GDC 973 considered
an important factor associated with food safety. This work clearly demonstrated that RpoN plays an important role in the resistance of C. jejuni to various stresses. Compared to the wild type, the rpoN mutant was more susceptible to osmotic stress (0.8% NaCl) and acidic pH. Interestingly, the rpoN mutation rendered C. jejuni more resistant to H2O2 than the wild type. Notably, the rpoN mutant exhibited significant survival defects in the static culture conditions. Although understanding of molecular mechanisms for stress tolerance may exceed the scope of our present work, in this study, we provided
new insights Nabilone into the role of RpoN, one of the three sigma factors of C. jejuni, in the survivability of this bacterial pathogen under various stress conditions. Methods Bacterial strains, plasmids, and culture conditions C. jejuni 81-176 was used in this study. The strains, plasmids, and primers used in this study are listed in Table 1. C. jejuni 81-176 and its derivatives were routinely grown at 42°C on MH agar plates or MH broth with shaking at 180 rpm under microaerobic condition (6% O2, 7% CO2, 4% H2, and 83% N2) adjusted by the MART (Anoxomat™, Mart Microbiology B.V, Netherlands). To investigate the effect of rpoN disruption on C. jejuni growth, C. jejuni was cultured in 50 ml MH broth either in conical tubes without shaking or in Erlenmeyer flasks with shaking. Occasionally, culture media were supplemented with kanamycin (50 μg ml-1) or chloramphenicol (10 μg ml-1) where required. Table 1 Bacterial strains, plasmids and primers used in this study Strains, plasmid and primers Description Source E. coli DH5α F’, Φ80 dlacZΔM15, endA1, recA1, hsdR17 (r k – , m k + ), supE44, thi1, Δ(lacZYA-argF)U169, deoR, λ – Invitrogen C.
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