The preceding study developed several intestinal mRNA biomarkers for P depletion in the rainbow trout although only sulfotrans ferase 2 was modified in the present study presumably because the target tissue was different. The results of the present study in the sea bream suggest that cytosolic sulfotransferase 2 may be a promising general marker of P depletion in fish and certainly merits further investigation. The effect of scale removal under food deprivation compared with food deprivation There was not such a pronounced effect as had been expected with scale removal and food deprivation and so an additional comparison was carried out between fasted animals and fasted animals with scales removed. Indeed, this comparison did produce the highest num ber of differentially expressed genes, 181 and 66 when the latter three treatments were com pared with control animals.
Surprisingly little overlap in significantly up regulated transcripts or modified net works were found between animals without scales and fasted animals Inhibitors,Modulators,Libraries without scales. The reason for this lack of overlap is diffi cult to explain but may result from asynchronous regen eration associated with the slow down in cellular metabolism and activation of alternative pathways to ensure barrier Inhibitors,Modulators,Libraries function when food is in short supply. This comparison provides the clearest signal of the sea bream response to scale removal with half of the 20 most up regulated annotated probes involved in cell division and mitosis. These probes are ideal candidates for the monitoring of cell division processes related to the regeneration of scales.
Of the remaining annotations, there are Entinostat representatives of cell growth and metabolism, cell proliferation, and cell signaling. The function of the multifunc tional ubiquitin in the present experiments remains to be elucidated Inhibitors,Modulators,Libraries as this gene has a large number of roles including cell cycle regulation, DNA repair, embry ogenesis, regulation of transcription and apoptosis. Interestingly, this comparative analysis may reveal the first hint of the start of mineralization processes. In par ticular mutations in the gene 3 beta hydroxysteroid delta8, delta7 isomerase which catalyses the conversion of delta sterols to their corresponding delta iso mers is linked to chondrodysplasia punctata in humans that causes punctiform calcification of cartilage.
It remains to be established if this gene also influences the Inhibitors,Modulators,Libraries calcifica tion process in fish but if it does it may represent a use ful biomarker. Moreover, it suggests that up regulation of transcripts involved in calcification occurs early in regeneration well before the most active phase of this process. The overexpression of developmental genes is already known to be involved in stem cell activation and in epidermal dermal interactions.
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