The production of these cytokines was Gefitinib determined from the coculture of the human monocytic cell line, THP-1, with either JFH-1-infected or uninfected hepatoma cells using the transwell system. Notably, JFH-1-infected HepG2 cells stimulated a statistically significant increase in the secretion of TGF-β, IL-6, IL-21, but not IL-12, by THP-1 cells in a transwell membrane system (Fig. 4C). However, cultures of THP-1 cells alone produced low levels of TGF-β, IL-6, and
IL-21 cytokines regardless of the presence of JFH-1sup (data not shown). Importantly, the addition of anti-TSLP-neutralizing antibodies led to a decrease of Th17-specific cytokines (Fig. 4C). These results suggest that monocytes/DCs conditioned by TSLP secreted from HCV-infected hepatocytes produce Th17 differentiating cytokines which could support the induction of CD4+ Th17 responses. Based on the role of IL-1, IL-6, and IL-21 production in Th17 polarization, we evaluated the effect of hepatocyte-derived TSLP on Th17 differentiation in coculture of naïve T cells with DCs activated by IL-1/IL-6/IL-23, JFH-1sup, or JFH-1sup plus anti-TSLP antibodies. Following stimulation with PMA/ionomycin, the production of intracellular cytokines (IFN-γ, IL-17A) by CD4+ T cells was assessed this website using flow cytometry. As expected, IL-1/IL-6/IL-23-treated DCs, used as positive control, produced more IL-17 cells compared to control cells (5.09 ± 0.6% versus 0.91 ± 0.08%). Notably, the percentage of IL-17-producing
cells increased after coculture of CD4+ T cells with JFH-1sup-treated DCs (4.65 ± 0.55%), which then significantly decreased upon the addition also of anti-TSLP mAbs (1.21 ± 0.1%) (Fig. 5A,B). There was
no significant difference in the percentage of IFN-γ production from JFH-1sup-treated DCs in the presence or absence of anti-TSLP antibody (Fig. 5A,B). This result was further verified by the detection of IL-17 release using ELISA. The enhancement of IL-17-producing T cells by JFH-1sup-treated DCs was significantly inhibited by neutralization of TSLP (Fig. 5C). This suggests that TSLP released from infected hepatocytes activates and conditions DCs to drive the differentiation of activated CD4+ T cells into Th17 cells. To further examine the effect of TSLP on promoting Th17 differentiation during HCV infection, we assessed the capacity of Th17 cell generation by CD4+ T cells from PBMC in a chronic HCV patient. As shown in Fig. 6A, there is a significant increase of Th17 lineage-specific transcription factor (i.e., RORc) and markers (i.e., CCR6 and CD161) from chronic HCV patients as compared to those in healthy individuals. We next determined the role of HCV-specific antigen in induction of Th17 CD4+ T cells. HCV NS3/5 proteins have been reported to induce a Th17 response.22 Th1/Th17 cells differentiations were compared using intracellular staining of IFN-γ and IL-17, respectively. The results indicated that Th17 cells were significantly increased in response to NS3/NS5 compared to normal control (5.
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