This approach should be ideally combined with other therapies able to target the aggressive hypoxia related undifferentiated subpopulation. All analyses MAPK Inhibitor Library involving human melanoma tissue were performed in accordance with the ethical committee in canton Zurich.
Immunohistochemistry was performed on three different tissue microarrays (TMAs) representing a total of 81 primary melanomas, 59 melanoma metastasis and 65 melanoma patients’ derived cell cultures. The TMAs partly included matched tumor samples from primary tumors, metastases and cell cultures. Totally, 9 triplets consisting of primary melanoma, metastases and cell cultures, 5 pairs including primary melanoma and metastases and 25 pairs of melanoma tissue (9
primary and 16 metastases) matched with cell cultures were analysed. One TMA consisted of primary melanomas (Breslow tumor thickness > 1 mm) with available clinical data and follow up information about the patients included. Detailed clinical information Ku-0059436 clinical trial of this TMA has been reported in a previous study [18]. The melanoma cells cultures were derived from surgical specimen of melanoma patients included in a life bio bank project. Written informed consent was approved by the local IRB (EK647 and EK800). TMA containing melanoma cell cultures and melanoma tissue were constructed as previously described [19]. Approval for the use of melanoma TMAs and melanoma metastases was obtained from the official ethical authorities of the Canton Zurich (StV 16–2007). All animal experiments were performed in accordance with Swiss law and
have been approved by the veterinary authorities of Zurich. For the mouse experiments: skin samples were fixed with Rebamipide 4% formaldehyde and frozen in OCT compound. For immunohistochemistry, sections were stained as previously described [20]. Anti-Dct (rabbit, ab74073, Abcam) was used. Sections of 2 μm from a tissue TMA were stained with antibodies against Melan A, Hif-1α, TRP-2 and Mib-1. The immunohistochemical staining for all antigens was performed on automated staining systems Melan A, TRP-2/Mib-1 on Ventana Bench Mark, Ventana Medical Systems, Tucson, AZ, USA and Hif-1α on Bond Refine, Vision BioSystems Ltd, Newcastle Upon Tyne, UK. The following antibodies were used: Hif-1α clone mgc3 (Abcam Limited), dilution 1:400; Melan A clone A103 (DAKO A/S), dilution 1:30; Mib-1 clone 30–9 (Ventana-Roche), prediluted. To determine the expression frequencies of TRP-2, the hot spot of a tumor sample was chosen and the percentage of positive cells per 100 melanoma cells was recorded. In addition, using a co-staining for Mib-1, four different combinations of positive and negative cells for Mib-1 and TRP-2 were recorded.
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