To study the association between pulmonary function and the SNPs in the ALOX5AP gene in a healthy and general population, the predicted values for forced expiratory volume in one second (FEV1; FEV1_%PRED) and the proportion of the FVC exhaled in the first second (FEV1/FVC; FEV1/FVC_PRED) in the KARE database were used. The 662 subjects with asthma, chronic lung disease (pneumoconiosis and silicosis), tuberculosis, or a previous diagnosis of respiratory-related
disease were excluded. In addition, 4553 subjects Opaganib supplier without diagnosis, treatment, FEV1, FEV1/FVC, height and smoking status information were also excluded. Therefore, 3627 subjects without respiratory disease were included and defined as a healthy population in this study. The average age of this population was 52.4 ± 8.9. The specific characteristics of Ansan and Ansung cohorts are described in Table 1. Total subjects including healthy population and with respiratory diseases or no information on medical history selleck compound were described as a general population in this study. Genotyping. The genotype data regarding the SNPs in the ALOX5AP gene, which are available to the research community through the KARE project from KoGES, were analysed. The study protocol was approved by the Institutional Review Board of KNIH. Affymetrix Genome-Wide Human SNP Array 5.0 (Affymetrix Inc., Santa Clara, CA, USA) was used to genotype the samples from the Ansan and Ansung
cohorts. The Bayesian Robust Linear Model with the Mahalanobis distance algorithm was used to determine the genotypes at each SNP of Affymetrix 5.0. The SNPs were excluded if any of the following criteria were met: (1) a call rate lower than 95%, (2) a minor allele frequency lower than 0.05 or Liothyronine Sodium (3) a significant deviation from the Hardy–Weinberg equilibrium that was lower than 0.05. Among SNPs filtered by the criteria, only tagging SNPs were used for all analysis in this study. Statistical analyses. The associations between the ALOX5AP SNPs and diplotypes of the 3627 subjects without asthma or respiratory
disease and the two pulmonary function measures FEV1 and FEV1/FVC were evaluated by performing a linear regression. A permutation test was performed for multiple comparisons in the association analysis. Interactions between SNPs in the ALOX5AP and smoking status on FEV1 and FEV1/FVC were analysed using linear regression. For the analysis of general population, 8535 subjects excluded 307 subjects without FEV1, FEV1/FVC, height and smoking status information was used for linear regression without consideration for the medical history of respiratory-related diseases. Only tagging SNPs were used for analysis. Every analysis on combined data was adjusted for residence area, sex, age, height and ever/never smoking status. All analysis on Ansung and Ansan data was adjusted for sex, age, height and ever/never smoking status.
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