We found support
for our hypothesis that D-Asp activated a mixed collection of receptors by the summary actions of many reagents. D-Asp may activate at least two different known ion currents in Aplysia BSC neurons: EAATs and NMDA-like receptors containing NR2-like subunits. The component of D-Asp current that is not due to NMDA-like receptors or EAAT current may represent a unique receptor channel. It is likely that D-Asp may substitute for L-Glu in eliciting Inhibitors,research,lifescience,medical excitation at some synapses, and potentially augment L-Glu responses at synapses possessing receptors for both agonists. Studies using cocultured synaptic preparations would greatly aid in characterization of the role of these agonists. Reduced preparations would be useful in informing the behavioral relevance of D-Asp. BSC neurons have receptive fields in the buccal region that undergo sensitization (Walters et al. 2004). Facilitation of D-Asp responses by serotonin (Carlson and Fieber Inhibitors,research,lifescience,medical 2011) may play a role in this, similar to facilitation of L-Glu in other characterized systems. Not greater than partial block Inhibitors,research,lifescience,medical of either L-Glu or D-Asp currents by L-GluR antJNK pathway inhibitors agonists suggests that neither Aplysia receptor is particularly well targeted by pharmacological agents developed for use in vertebrates. Ultimately, only molecular description
in cells preferentially expressing unique Aplysia D-Asp receptors will definitively identify Inhibitors,research,lifescience,medical the receptor. To date, however, the NR1 subunits
are the sole NMDARs to have been cloned from Aplysia (Ha et al. 2006). This study and Carlson and Fieber (2011) strongly support the findings of Errico et al. (2011) that D-Asp is a neurotransmitter at dedicated receptors in multiple species. We have summarized the effects of the known L-Glu antagonists to support the conclusion that D-Asp activates a receptor distinct from L-GluRs. Inhibitors,research,lifescience,medical Acknowledgments The authors gratefully acknowledge the staff of the University of Miami Aplysia Resource. This study is funded by NIH P40RR01029, the Korein Foundation, a University of Miami Fellowship to S. L. C. and a Maytag Fellowship to A. T. K.
Current guidelines recommend the use of cranial computed tomography (CCT) as a routine diagnostic procedure in the evaluation old of a transient ischemic attack (TIA) (Johnston et al. 2006; ESO 2008; Easton et al. 2009). Although evidence supporting the use of CCT to detect an infarct in patients suffering from a TIA is sparse, CCT is a mandatory part of clinical practice in the management of patients with acute stroke in emergency departments. A 10-year analysis found that 56% of patients suffering from a TIA who presented to an emergency department underwent CCT (Edlow et al. 2006). In a clinical trial by Koudstaal et al., new ischemic lesions were detected by CCT in 13% of TIAs (Koudstaal et al. 1992). A study by Douglas et al.
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