The screening process and inclusion criteria were quite restrictive. Thus, the sample size of our study is small, and this may limit our conclusions. Furthermore, an appropriate control group is lacking who underwent ‘sham – immunoadsorption therapy’. In our small control group of patients who refused IA therapy,
we postulated to examine changes in cellular immunity during progression of the disease, but we cannot verify this topic. We cannot rule out confounding (and yet unknown) factors that might have influenced cell-mediated immunity and benefit Ganetespib in vivo of IA. Furthermore, we did not analyse the auto-antibody status in our patients. So we cannot rule out confounding factors that (1) antibodies’ levels may influence our results and (2) patients with ischaemic cardiomyopathy may have auto-antibodies against myocardial targets. We did not examine whether patients with ischaemic cardiomyopathy would benefit of IA too. IA was performed as described previously by several investigators [5, 6, 12]. In these protocols, IA was followed by substitution of polyclonal immunoglobulins. Selleckchem Palbociclib We cannot disclose confounding factors of IG substitution, which may interact with cellular immunity.
Different ways are known to analyse Tregs. Tregs are broadly classified into natural Tregs (CD4+CD25+), which emigrate from the thymus to perform the key role in immune homoeostasis, or adaptive Tregs (non-regulatory CD4+ T cells),which acquire CD25 expression outside of the thymus. They are typically induced by autoimmunity [33]. Recently, the transcription factor forkhead box p3 (FOXP3) has been reported to play a major role in CD4+ CD25+ Treg function and represents a specific marker for these cells. However, FOXP3 is a nuclear protein and is of limited value in the isolation of Tregs, which is a major reason that many functionally relevant aspects of Treg cells are still unknown [34]. In this work, we did not analyse FOXP3. In
addition to cellular aspects, we did not analyse genetic polymorphisms in Fcγ-Receptor IIa as it was described previously [35]. This work was supported by a research grant from Fresenius Medical Care, mafosfamide Bad Homburg, Germany. Our group examined for the first time to our knowledge T cell subgroups in immunoadsorption in patients with dilated cardiomyopathy [12]. The actual study population was recruited after the publication of above-mentioned work. So none of the patients in this work was included in the previous work. “
“We investigated cellular immune responses at baseline in peripheral blood mononuclear cells (PBMC) of patients with multiple sclerosis (MS) treated with interferon (IFN)-β and classified into responders and non-responders according to clinical response criteria.
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- Second, because rather restrictive inclusion criteria were design
- Of these, 65 met the initial screening criteria and were sent on