(2007) observed that 40% of biofilm-producing strains showed the aggR gene vs. 11% of the nonbiofilm producers (P=0.008). In our study, this tendency
could be observed but without statistical significance. The presence of AggR was also related to chronic renal insufficiency, which could be due to the function of this transcriptional factor regulating adherence factors that allow the bacteria to colonize and to persist in the kidney. In conclusion, this is the first study on the presence of enterotoxins from Shigella and EPEC collected from blood. ShET-1 and EAST-1 have previously been found in E. coli but not in ShET-2. In addition, a relationship between quinolone resistance and the presence of the ShET-1 toxin has been demonstrated, although further studies Venetoclax nmr are needed to determine whether quinolones induce this excision. This work was supported by the projects FIS05/0068 of Fondo de Investigaciones Sanitarias of the Ministry of Health, the Spanish Network for the Research in Infectious Diseases (REIPI RE06/0008) and SGR050444 from the Departmanet d’Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya, Spain. S.M.S. is recipient of a contract from the ‘Sistema Nacional de Salud’ (CP05/00140) from Fondo de Investigaciones Sanitarias from the Ministry of Health of Spain. This work has also been supported
by funding from the European Community (TROCAR contract HEALTH-F3-2008-223031). M.T. has a fellowship from Federation of European Microbiological Societies. “
“Development of bacteriophage T4 depends on the physiological state of Pifithrin-�� cell line its host cell. Based on previous studies performed under
laboratory conditions with different media determining various growth rates of Escherichia coli, a mathematical model was developed which suggested that phage T4 development cannot proceed efficiently in bacteria growing with a doubling time longer than 160 min. Contrary to this prediction, using a chemostat culture system allowing for culturing E. coli at different growth rates without changes in the medium ADP ribosylation factor composition, we found that T4 can yield progeny in host cells growing with a doubling time as long as 21 h. Our results indicate that the actual limiting growth rate of the host culture for the development of phage T4 is about 0.033 h−1, corresponding to the doubling time of about 21 h. “
“MicroRNAs (miRNAs) are important modulators of gene expression in eukaryotic cells. However RNAs of the same size in bacteria have not been specifically discussed previously. Here, we provide a library of miRNA-size RNAs (msRNAs), which were registered by deep sequencing in Streptococcus mutans. Bioinformatic analysis of the whole set revealed more than 900 individual msRNA species. The cellular content of selected msRNAs was verified by quantitative RT-PCR and Northern blotting.
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