It can also be observed that the fat content (FAT) and partial fat coalescence (PFC) had a strong negative correlation with Factor 2, while the overrun (OVE) and the melting rate (MR) were positively correlated with this factor ( Fig. 4). The enzymatic treatment with TG of the ice cream samples with 4, 6 and 8 g/100 g fat led to an increase in the overrun, partial coalescence of fat globules, melting resistance and hardness compared
with the samples without enzyme treatment. Regarding the rheological parameters, protein polymerization induced by TG favored the pseudoplastic properties of the ice cream and gave higher values for the apparent viscosity, consistency index, hysteresis and initial tension required to initiate the structural break of the samples. The addition of transglutaminase Daporinad in vitro led to the ice cream samples with 4 g/100 g and 8 g/100 g fat having similar characteristics, indicating that the enzyme can be used as a partial replacement for fat in ice cream, without affecting the functional and rheological properties of these products. The authors
are grateful to the Conselho Nacional de Pesquisa (CNPq) for the financial support and also to the company Ajinomoto Co., Inc. for providing the transglutaminase preparations. “
“The various health benefits of probiotic bacteria mainly belonging to the Lactobacillus and Bifidobacterium genera have led to their increased incorporation in yoghurts and fermented milks ( Ashraf & Shah, 2011), ice creams and Dabrafenib mouse pharmaceutical products ( Mattila-Sandholm et al., 2002). To enhance their therapeutic effects, dairy foods usually contain also prebiotics, i.e. non-digestible oligosaccharides that resist hydrolysis and absorption in the upper gastrointestinal tract and are metabolized selectively by at least
one type of probiotic in the colon (Mattila-Sandholm et al., 2002). Among these, inulin was shown to exert a protective effect on lactic acid bacteria (LABs) by stimulating their survival and activity during storage of the final product (Donkor, Nilmini, Stolic, Vasiljevic, & Shah, 2007). It is a soluble and fermentable fructan that cannot be digested by α-amylase or other Progesterone hydrolytic enzymes (Villegas & Costell, 2007) and is mainly applied to get low-fat products (Oliveira, Florence et al., 2009). Although the metabolic response of homofermentative and heterofermentative LABs to environmental conditions is well documented (Axelsson, 1998), there is scarce information on the metabolism of probiotics in co-cultures. Among LABs, lactobacilli are classified as gram-positive, non-sporulating, catalase-negative, acid-tolerant, anaerobic fermentative bacteria with different sensitivity to oxygen and a reputed Generally Recognized as Safe (GRAS) status (Kleerebezem & Hugenholtz, 2003).
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