BMC Microbiol 2012,12(1):214.PubMedCrossRef 101. Chang T, Yao S: Thermophilic, lignocellulolytic bacteria for ethanol production: current state and perspectives. Appl Microbiol Biotechnol 2011,92(1):13–27.PubMedCrossRef 102. Guedon E, Desvaux
M, Petitdemange H: Improvement of cellulolytic properties of Clostridium cellulolyticum HKI272 by metabolic engineering. Appl Environ Microbiol 2002,68(1):53–58.PubMedCrossRef 103. Tripathi SA, Olson DG, Argyros DA, Miller BB, Barrett TF, Murphy DM, McCool JD, Warner AK, Rajgarhia VB, Lynd LR, et al.: Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant. Appl Environ Microbiol 2010,76(19):6591–6599.PubMedCrossRef Authors’ contributions TR and CRC co-authored the manuscript. TV, CRC and TR performed genomic meta-analysis. TR performed end-product comparisons and thermodynamic calculations. CRC performed phylogenetic
analysis. RS, NC, and DBL conceived of the study, participated in its design, and helped draft the manuscript. All authors read and approved the final manuscript.”
“Background The genus Arcobacter, included in the family Campylobacteraceae, find more has expanded rapidly since it was first recognised in 1991 [1], and currently includes 17 species. Some of these species are considered enteropathogenic to humans and animals, as well as important zoonotic agents. Arcobacter species negatively impact the food industry, as many meat products are frequently contaminated with these bacteria, and multiple species Parvulin have been described from shellfish [2–6]. In addition, the International Commission on Microbiological
Specification for Foods classified A. butzleri as a serious hazard to human health [7]. However, the true incidence of Arcobacter species in environmental and clinical samples is thought to be underestimated because specific detection and identification methods are not normally applied and can be inaccurate [2, 8]. A 16S rRNA restriction fragment length polymorphism (RFLP) buy XAV-939 method for the identification of Arcobacter species has previously been described [9]. The method involved a single digestion with the MseI endonuclease and discriminated all Arcobacter species that had been described up to 2008, i.e. A. butzleri, A. cryaerophilus, A. cibarius, A. skirrowii, A. nitrofigilis and A. halophilus[9]. Further molecular methods for the identification of Arcobacter species have been reviewed elsewhere [2, 9]. Most of the methods described target only the most common species i.e. A. butzleri[10, 11], A. cryaerophilus[12] and/or A. skirrowii[13, 14]. Even the most recently proposed identification method, m-PCR, described by Douidah et al. [15] in 2010, only targeted five species: A. butzleri, A. cryaerophilus, A. skirrowii, A. cibarius and A. thereius.
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