Error bars reflect ± SEM (based on variation between 6 adults per treatment group). Differences were considered significant at (***) p < 0.001 for total 16S rDNA copy numbers of placebo vs. other antibiotic treated zebrafish in each intestinal tissue analyzed. Impact of antibiotic exposure on expression of the tra genes of pRAS1 The expression of traD, virB11 and virD4 was strongly induced by ineffective treatment (tetracycline, trimethoprim and sub-inhibitory levels of flumequine) and strongly reduced by treatment with effective concentrations of flumequine OSI906 [Figure 4]. However, ineffective sulphonamide slightly reduced the expression of these genes. Figure 4 Expression of three pRAS1 plasmid mobility genes
in intestinal samples from adult zebrafish 48 h post treatment (72 h post experimental infection) relative to placebo treatment. Error bars represent ± SEM (based on variation between 6 adults per treatment group). Differences were considered significant at (*) p < 0.05, (**) p < 0.01 and (***) p < 0.001 for mobility gene expression levels of tetracycline vs. other antibiotic treated zebrafish in each intestinal tissue analyzed. Immune responses following effective and ineffective
treatments Our results revealed a strong up-regulation of all four analyzed immune related genes after effective https://www.selleckchem.com/products/DAPT-GSI-IX.html flumequine treatment. An induction of some of these genes was observed even after ineffective treatment with trimethoprim, sulphonamide and a sub-lethal level of flumequine, whereas ineffective tetracycline treatment apparently suppressed two of the innate immune response mediators [Figure 5]. Figure 5 Expression of selected inflammatory and immune response genes in the entire intestine of experimentally infected zebrafish 48 h post antibiotic treatment, relative to the expression in placebo treated fish (ref. Figure 2). Error bars represent ± SEM (based on variation between 6 adults per treatment group). Differences were considered significant at (*) p < 0.05, (**) p < 0.01 and (***) p < 0.001 for immune response levels of tetracycline vs. other antibiotic treated zebrafish in each intestinal tissue analyzed. Discussion In this study, we have for the first
time employed an experimental Interleukin-3 receptor zebrafish infection- treatment model to mimic the conditions under which antibiotic resistance (mediated by a naturally occurring R-plasmid) transfer takes place in the intestinal microbiota during an infection caused by a resistant pathogen treated with effective or ineffective antibiotic treatments. We were able to establish an infection with A. hydrophila resulting in disease symptoms similar to those previously described [10, 11] but with no mortality 3 days post- infection, as intended in our study design. Rodriguez et al. [10] and Pullium et al. [11] observed per-acute cases of A. hydrophila infection with high mortality rates within a few hours possibly related to intraperitoneal injection of bacterial extracellular toxins and/or enzymes.
Related posts:
- Recently, DGE and microarray based transcriptome profiling rese
- The conflict effect in error rate can be predicted by the conflic
- The conflict effect in error rate can be predicted by the conflic
- 0 fold and 11 8 fold, respectively The decrease of apolipoprote
- Immunofluorescence staining was performed by using red fluorescen