s., Calobota and Wiborgiella [7]. The Lebeckia s.s. section, which includes L. ambigua, can easily be distinguished from other species by their acicular leaves and 5+5 anther arrangement [7-9]. In four expeditions to the Western Cape of South during Africa, between 2002 and 2007, nodules and seeds of Lebeckia ambigua were collected and stored [5]. The isolation of RNB from these nodules gave rise to a collection of 23 microsymbionts that clustered into five groups within the genus Burkholderia [5]. Unlike most of the previously studied rhizobial Burkholderia strains, this South African group appears to be associated with papilionoid forage legumes (rather than Mimosa spp.). One of these Burkholderia strains has now been designated as the type strain of the new species ��Burkholderia sprentiae�� strain WSM5005T [10].
This isolate effectively nodulates Lebeckia ambigua and L. sepiaria [5]. Here we present a summary classification and a set of general features for ��Burkholderia sprentiae�� strain WSM5005T together with the description of the complete genome sequence and its annotation. Classification and general features ��Burkholderia sprentiae�� strain WSM5005T is a motile, Gram-negative, non-spore-forming rod (Figure 1, left and center panels) in the order Burkholderiales of the class Betaproteobacteria [10]. It is fast growing, forming 2-4 mm diameter colonies within 2-3 days when grown on half Lupin Agar (?LA) [11] at 28��C. Colonies on ?LA are white-opaque, slightly domed, moderately mucoid with smooth margins (Figure 1, right panel).
Figure 1 Images of ��Burkholderia sprentiae�� strain WSM5005T using scanning (Left) and transmission (Center) electron microscopy and the colony morphology on a solid medium (Right). Minimum Information about the Genome Sequence (MIGS) is provided in Table 1. Figure 2 shows the phylogenetic relationship of ��Burkholderia sprentiae�� strain WSM5005T in a 16S rRNA sequence based tree. This strain clusters closest to Burkholderia tuberum STM678T (CIP 108238T) and Burkholderia kururiensis KP23T with 98.2% and 96.9% sequence identity, respectively. Table 1 Classification and general features of ��Burkholderia sprentiae�� strain WSM5005T according to the MIGS recommendations [12,13]. Figure 2 Phylogenetic tree showing the relationships of ��Burkholderia sprentiae�� strain WSM5005T (shown in blue print) with some of the bacteria in the order Burkholderiales based on aligned sequences of the 16S rRNA gene (1,322 bp internal region).
… Symbiotaxonomy ��Burkholderia sprentiae�� strain WSM5005T is part of a cadre of Burkholderia strains that were assessed for nodulation Cilengitide and nitrogen fixation on three separate L. ambigua genotypes (CRSLAM-37, CRSLAM-39 and CRSLAM-41) and on L. sepiaria [5]. Representatives of this group of nodule bacteria are generally Nod+ and Fix- on Macroptillium atropurpureum and appear to have a very narrow host range for symbiosis.
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